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Cell No. : Cell Name
RCB2614 : KLN205-MUC1  update : 2021/05/20
CommentMouse cell line derived from lung squamous cell carcinoma. TKG0473 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kaneko, T.
Year of deposit 2007
Original cell TKG0473
Animal mouse < Mammals
Strain name DBA/2
Tissue lung
Disease name squamous cell carcinoma
Classification cancer
Recombinant recombinant
Exogene MUC1
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0473)
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8725
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
5262  Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.  Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.  J Cell Sci  1991  100 ( Pt 4):841-9  PubMed ID: 1814933  
5197  Kaneko T, LePage GA, Shnitka TK.  KLN205--a murine lung carcinoma cell line.  In Vitro  1980  16(10):884-92  PubMed ID: 7419233  
5196  Kaneko T, LePage GA.  Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo.  Cancer Res  1978  38(7):2084-90  PubMed ID: 418873  

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User's Publication
16208  oshihiro Uchihashi, Hirokazu Nakahara, Hiroshi Fukuhara, Miwako Iwai, Hirotaka Ito, Akinari Sugauchi, Minoru Tanaka, Mikihiko Kogo, Tomoki Todo   Oncolytic herpes virus G47Δ injected into tongue cancer swiftly traffics in lymphatics and suppresses metastasis  Mol Ther Oncolytics  2021  22:388-398  PubMed ID: 34553027   DOI: 10.1016/j.omto.2021.06.008



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