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Cell No. : Cell Name
RCB2611 : MDCK-AD  update : 2022/05/30
CommentDog cell line derived from kidney.
Comment from the depositor
Terms and conditionsThe BIOLOGICAL RESOURCE used research only.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kobayashi, Yukiharu
Originator Irie, Hiroshi
Year of deposit 2007
Animal dog < Mammals
Genus Canis
Species familiaris
Tissue kidney
Classification other
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_K177
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
deposit info
lot info
Reference information Reference 1
User's Publication 0

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260  Gaush CR, Hard WL, Smith TF.  Characterization of an established line of canine kidney cells (MDCK)  Proc Soc Exp Biol Med.  1966  122(3):931-5  PubMed ID: 5918973  

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