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Cell No. : Cell Name
RCB2610 : FELV3281-AD  update : 2024/10/02
CommentBSL2. Cat cell line derived from lymphoma. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2.
Comment from the depositor
Terms and conditionsThe BIOLOGICAL RESOURCE used research only.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   Cell line to be handled at BSL 2(C-0070.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kobayashi, Yukiharu
Originator Takayama, Katsuyoshi
Year of deposit 2007
Original cell FeLV3281
Animal _cat < Mammals
Genus Felis
Gender Unknown
Tissue lymphoma
Disease name Feline leukemia virus (FeLV)
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
BSL BSL2
Cellosaurus(Expasy) CVCL_8689
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
10383  Fujita M, Kaneda M.  DNA methylation inhibitor causes cell growth retardation and gene expression changes in feline lymphoma cells.  J. Vet. Med. Sci.  2017  79:1352-1358  PubMed ID: 28652519   DOI: 10.1292/jvms.17-0179



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