Cell No. : Cell Name
RCB2610 : FELV3281-AD
update : 2025/06/12
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| Comment | BSL2. Cat cell line derived from lymphoma. We don't know whether virus particles are produced or not. Therefore, the cells must be handled under BSL2. |
| Comment from the depositor | |
| Terms and conditions | The BIOLOGICAL RESOURCE used research only. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
Cell line to be handled at BSL 2(C-0070.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Kobayashi, Yukiharu
|
| Originator |
Takayama, Katsuyoshi
|
| Year of deposit |
2007
|
| Original cell |
FeLV3281
|
| Animal |
_cat
< Mammals
|
| Genus |
Felis
|
| Gender |
Unknown
|
| Tissue |
lymphoma
|
| Disease name |
Feline leukemia virus (FeLV)
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| Classification |
cancer
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|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| BSL |
BSL2
|
| Cellosaurus(Expasy) |
CVCL_8689
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| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 10 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| DNA barcoding |
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OK
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| Isozyme |
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LD, NP, AST
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
2
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