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Cell No. : Cell Name
RCB2541 : 141-LCL-MUC1  update : 2021/05/20
CommentHuman cell line derived from B cells of healthy volunteer. Transformed by EB virus. RCB2083 was derived from the same patient. TKG0634 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Fukuda, Hiroshi
Originator Kudo, T.
Year of deposit 2007
Original cell TKG0634, TKG0611 141-LCL
Animal human < Mammals
Genus Homo
Species sapiens
Tissue peripheral blood
Disease name EBV-transformed lymphoblast cell
Classification transformed
Recombinant recombinant
Exogene APR-MUC1 cDNA
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0499)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8071
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 200μg/ml G418
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 0


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Reference
5262  Batra SK, Kern HF, Worlock AJ, Metzgar RS, Hollingsworth MA.  Transfection of the human Muc 1 mucin gene into a poorly differentiated human pancreatic tumor cell line, Panc1: integration, expression and ultrastructural changes.  J Cell Sci  1991  100 ( Pt 4):841-9  PubMed ID: 1814933

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