Cell No. : Cell Name RCB2534 : M-MOK  update : 2024/09/10
Comment			Human cell line derived from megakaryoblastic leukemia. TKG0458 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Fukuda, Hiroshi
		Originator	Itano, Masataka
		Year of deposit	2007
		Original cell	TKG0458
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Female
		Tissue	bone marrow
		Disease name	immature megakaryoblastic leukemia
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0458）
		Lifespan	infinite
		Morphology	lymphoblast-like
		Cellosaurus(Expasy)	CVCL_8731
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture method		浮遊細胞の培養に関する一般的な注意(Japanese)
		Culture medium		RPMI1640 + 10% FBS + 10ng/ml GM-CSF
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	2
		User's Publication	2

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Reference

5195  Morita S1, Tsuchiya S, Fujie H, Itano M, Ohashi Y, Minegishi M, Konno T.  Isolation and characterization of two monoclonal antibodies that recognize different epitopes of the human c-kit receptor.  Tohoku J Exp Med  1996  178(2):187-98  PubMed ID: 8727700   DOI: 10.1620/tjem.178.187

5194  Itano M, Tsuchiya S, Minegishi N, Fujie H, Minegishi M, Morita S, Yambe T, Ohashi Y, Masuda T, Koike T, et al.  Establishment and characterization of a novel human immature megakaryoblastic leukemia cell line, M-MOK, dependent on fibroblasts for its viability.  Exp Hematol  1995  23(12):1301-9  PubMed ID: 7589286

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User's Publication

21650  Matsuo H, Inagami A, Ito Y, Ito N, Iyoda S, Harata Y, Higashitani M, Shoji K, Tanaka M, Noura M, Mikami T, Kato I, Takita J, Nakahata T, Adachi S.  Parbendazole as a promising drug for inducing differentiation of acute myeloid leukemia cells with various subtypes.  Commun Biol  2024  7(1):123  PubMed ID: 38267545   DOI: 10.1038/s42003-024-05811-8

11559  Drenberg CD, Shelat A, Dang J, Cotton A, Orwick SJ, Li M, Jeon JY, Fu Q, Buelow DR, Pioso M, Hu S, Inaba H, Ribeiro RC, Rubnitz JE, Gruber TA, Guy RK, Baker SD.  A high-throughput screen indicates gemcitabine and JAK inhibitors may be useful for treating pediatric AML.  Nat Commun  2019    PubMed ID: 31097698   DOI: 10.1038/s41467-019-09917-0