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Cell No. : Cell Name
RCB2534 : M-MOK  update : 2020/09/24
CommentHuman cell line derived from megakaryoblastic leukemia. TKG0458 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Itano, Masataka
Year of deposit 2007
Original cell TKG0458
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue bone marrow
Case history immature megakaryoblastic leukemia
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0458)
Lifespan infinite
Morphology lymphoblast-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 10ng/ml GM-CSF
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
5195  Morita S1, Tsuchiya S, Fujie H, Itano M, Ohashi Y, Minegishi M, Konno T.  Isolation and characterization of two monoclonal antibodies that recognize different epitopes of the human c-kit receptor.  Tohoku J Exp Med  1996  178(2):187-98  PubMed ID: 8727700
5194  Itano M, Tsuchiya S, Minegishi N, Fujie H, Minegishi M, Morita S, Yambe T, Ohashi Y, Masuda T, Koike T, et al.  Establishment and characterization of a novel human immature megakaryoblastic leukemia cell line, M-MOK, dependent on fibroblasts for its viability.  Exp Hematol  1995  23(12):1301-9  PubMed ID: 7589286

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User's Publication
11559  Drenberg CD, Shelat A, Dang J, Cotton A, Orwick SJ, Li M, Jeon JY, Fu Q, Buelow DR, Pioso M, Hu S, Inaba H, Ribeiro RC, Rubnitz JE, Gruber TA, Guy RK, Baker SD.  A high-throughput screen indicates gemcitabine and JAK inhibitors may be useful for treating pediatric AML.  Nat Commun  2019    PubMed ID: 31097698



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