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Cell No. : Cell Name
RCB2532 : HuO 9N2 (O9N2)  update : 2023/10/16
CommentHuman cell line derived from osteosarcoma. TKG0451 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Fukuda, Hiroshi
Originator Kawai, A.
Year of deposit 2007
Original cell TKG0451
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue bone
Disease name osteosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0451)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2958
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 3
User's Publication 6

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5194  Itano M, Tsuchiya S, Minegishi N, Fujie H, Minegishi M, Morita S, Yambe T, Ohashi Y, Masuda T, Koike T, et al.  Establishment and characterization of a novel human immature megakaryoblastic leukemia cell line, M-MOK, dependent on fibroblasts for its viability.  Exp Hematol  1995  23(12):1301-9  PubMed ID: 7589286  
5193  Kawai A.  A newly established human osteosarcoma cell line with osteoblastic properties.  Clin Orthop Relat Res  1990  256-67  PubMed ID: 2170070  
5192  Kawai A, Ozaki T, Ikeda S, Oda T, Miyazaki M, Sato J, Taketa K, Inoue H.  Two distinct cell lines derived from a human osteosarcoma.  J Cancer Res Clin Oncol  1989  115(6):531-6  PubMed ID: 2691514   DOI: 10.1007/bf00391353

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User's Publication
13409  Hirozane T, Masuda M, Sugano T, Sekita T, Goto N, Aoyama T, Sakagami T, Uno Y, Moriyama H, Sawa M, Asano N, Nakamura M, Matsumoto M, Nakayama R, Kondo T, Kawai A, Kobayashi E, Yamada T.  Direct conversion of osteosarcoma to adipocytes by targeting TNIK.  JCI Insight  2021    PubMed ID: 33400690   DOI: 10.1172/jci.insight.137245
13419  Suzuki S, Yuan H, Hirata-Tsuchiya S, Yoshida K, Sato A, Nemoto E, Shiba H, Yamada S.  DMP-1 promoter-associated antisense strand non-coding RNA, panRNA-DMP-1, physically associates with EGFR to repress EGF-induced squamous cell carcinoma migration.  Mol Cell Biochem  2021    PubMed ID: 33420898   DOI: 10.1007/s11010-020-04046-5
10919    Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma  Integr Mol Med  2016  3: 755-760    DOI: 10.15761/IMM.1000239
6427  Kobayashi E, Satow R, Ono M, Masuda M, Honda K, Sakuma T, Kawai A, Morioka H, Toyama Y, Yamada T.  MicroRNA expression and functional profiles of osteosarcoma.  Oncology  2014  86(2):94-103  PubMed ID: 24457375   DOI: 10.1159/000357408
6431  Shibuya H, Hamamura K, Hotta H, Matsumoto Y, Nishida Y, Hattori H, Furukawa K, Ueda M, Furukawa K.  Enhancement of malignant properties of human osteosarcoma cells with disialyl gangliosides GD2/GD3.  Cancer Sci  2012  103(9):1656-64  PubMed ID: 22632091   DOI: 10.1111/j.1349-7006.2012.02344.x
18251  Rouleau C, Smale R, Fu YS, Hui G, Wang F, Hutto E, Fogle R, Jones CM, Krumbholz R, Roth S, Curiel M, Ren Y, Bagley RG, Wallar G, Miller G, Schmid S, Horten B, Teicher BA.  Endosialin is expressed in high grade and advanced sarcomas: evidence from clinical specimens and preclinical modeling  Int J Oncol  2011  39(1):73-89  PubMed ID: 21537839   DOI: 10.3892/ijo.2011.1020

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