戻る 戻る
細胞番号 : 細胞名
RCB2530 : A172  update : 2023/10/16
細胞特性(Comment:英)Human cell line derived from glioblastoma. TKG0183 (Deposited from Tohoku Univ.).
細胞特性(日)ヒト神経膠芽腫(Glioblastoma)由来細胞株。 TKG0183 (東北大学医用細胞資源センターからの寄託)。
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Fukuda, Hiroshi
樹立者 Giard, D. J.
寄託日 2007
元の細胞 TKG0183
動物種 _human < Mammals
属名 Homo
種名 sapiens
採取組織 brain
病名 glioblastoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0183)
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_0131
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
個体識別検査 OK
文献情報 Reference(英) 1件
Reference(日) 0件
利用者成果(英) 34件
利用者成果(日) 0件

1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758   DOI: 10.1093/jnci/51.5.1417


21718  Kudo K, Yanagiya R, Hasegawa M, Carreras J, Miki Y, Nakayama S, Nagashima E, Miyatake Y, Torii K, Ando K, Nakamura N, Miyajima A, Murakami M, Kotani A.  Unique lipid composition maintained by extracellular blockade leads to prooncogenicity.  Cell Death Discov  2024  10(1):221  PubMed ID: 38719806   DOI: 10.1038/s41420-024-01971-y
19770  Mitobe Y, Suzuki S, Nakagawa-Saito Y, Togashi K, Sugai A, Sonoda Y, Kitanaka C, Okada M.  Antagonizing MDM2 Overexpression Induced by MDM4 Inhibitor CEP-1347 Effectively Reactivates Wild-Type p53 in Malignant Brain Tumor Cells  Cancers (Basel)  2023  15(17):4326  PubMed ID: 37686602   DOI: 10.3390/cancers15174326
21053  Kakuo M, Horii T, Tonomura N, Sato R, Ogawa M, Okajima T, Kamemura K  Evidence that only EWS among the FET proteins acquires a low partitioning property for the hyperosmotic stress response by O-GlcNAc glycosylation on its low-complexity domain.  Exp Cell Res  2023  424(1):113504  PubMed ID: 36736606   DOI: 10.1016/j.yexcr.2023.113504
20548  Tamura Y, Nakamizo Y, Watanabe Y, Kimura I, Katoh H.  Filamin A forms a complex with EphA2 and regulates EphA2 serine 897 phosphorylation and glioblastoma cell proliferation.  Biochem Biophys Res Commun  2022  597:64-70  PubMed ID: 35124461   DOI: 10.1016/j.bbrc.2022.01.050
20564  Hayashima K, Katoh H.  Expression of gamma-glutamyltransferase 1 in glioblastoma cells confers resistance to cystine deprivation-induced ferroptosis.  J Biol Chem  2022  101703  PubMed ID: 35148992   DOI: 10.1016/j.jbc.2022.101703
20718  Ando S, Kojima N, Moyama C, Fujita M, Ohta K, Ii H, Nakata S.  JCI-20679 suppresses the proliferation of glioblastoma stem cells by activating AMPK and decreasing NFATc2 expression levels.  Mol Med Rep  2022  26(1)  PubMed ID: 35621135   DOI: 10.3892/mmr.2022.12754
20874  Mitobe Y, Nakagawa-Saito Y, Togashi K, Suzuki S, Sugai A, Matsuda KI, Sonoda Y, Kitanaka C, Okada M.  CEP-1347 Targets MDM4 Protein Expression to Activate p53 and Inhibit the Growth of Glioma Cells.  Anticancer Res  2022  42(10):4727-4733  PubMed ID: 36192008   DOI: 10.21873/anticanres.15977
13413  Hayashima K, Kimura I, Katoh H.  Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells.  Biochem Biophys Res Commun  2021    PubMed ID: 33421769   DOI: 10.1016/j.bbrc.2020.12.075
12999  Kinashi Y, Ikawa T, Takahashi S.  The combined effect of neutron irradiation and temozolomide on glioblastoma cell lines with different MGMT and P53 status.  Appl Radiat Isot  2020    PubMed ID: 32561044   DOI: 10.1016/j.apradiso.2020.109204
14294  Soeta K, Iuchi K, Hisatomi H, Yokoyama C.  Generation of Rat Monoclonal Antibody for Cytokeratin 18 by Immunization of Three-Dimensional-Cultured Cancer Cells  Monoclon Antib Immunodiagn Immunother  2020  39(6):199-203  PubMed ID: 33064594   DOI: 10.1089/mab.2020.0030
11332  Toyama M, Hamaoka Y, Katoh H.  EphA3 is up-regulated by epidermal growth factor and promotes formation of glioblastoma cell aggregates.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30528229   DOI: 10.1016/j.bbrc.2018.12.002
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217   DOI: 10.1016/j.bbrep.2019.100632
11581  Teramoto K, Katoh H.  The cystine/glutamate antiporter xCT is a key regulator of EphA2 S897 phosphorylation under glucose-limited conditions.  Cell. Signal.  2019    PubMed ID: 31152846   DOI: 10.1016/j.cellsig.2019.05.014
11624  Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.  Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.  Viruses  2019    PubMed ID: 31288421   DOI: 10.3390/v11070626
11639  Taniguchi K, Ii H, Kageyama S, Takagi H, Chano T, Kawauchi A, Nakata S.  Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK-FOXO3a-p21 axis.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31345573   DOI: 10.1016/j.bbrc.2019.07.049
10522  Haryu S, Saito R, Jia W, Shoji T, Mano Y, Sato A, Kanamori M, Sonoda Y, Sampetrean O, Saya H, Tominaga T.  Convection-enhanced delivery of sulfasalazine prolongs survival in a glioma stem cell brain tumor model.  J. Neurooncol.  2018  136:23-31  PubMed ID: 28929335   DOI: 10.1007/s11060-017-2621-7
10677  Yaekashiwa N, Otsuki S, Hayashi S, Kawase K.  Investigation of the non-thermal effects of exposing cells to 70-300 GHz irradiation using a widely tunable source.  J. Radiat. Res.  2018  59:116-121  PubMed ID: 29281029   DOI: 10.1093/jrr/rrx075
4071  Zhiwei Qiao, Tadashi Kondo  Identification of cephalomannine as a drug candidate for glioblastoma via high-throughput drug screening  Journal of Electrophoresis  2018  62:17    DOI: 10.2198/jelectroph.62.17
4125  Hamaoka Y, Negishi M, Katoh H.  Tyrosine kinase activity of EphA2 promotes its S897 phosphorylation and glioblastoma cell proliferation.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 29626472   DOI: 10.1016/j.bbrc.2018.04.020
9963  Kamemura K.  O-GlcNAc glycosylation stoichiometry of the FET protein family: only EWS is glycosylated with a high stoichiometry.  Biosci. Biotechnol. Biochem.  2017  81:541-546  PubMed ID: 27903134   DOI: 10.1080/09168451.2016.1263148
10559  Goji T, Takahara K, Negishi M, Katoh H.  Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation.  J. Biol. Chem.  2017  292:19721-19732  PubMed ID: 29038291   DOI: 10.1074/jbc.M117.814392
7074  Hamaoka Y, Negishi M, Katoh H.  EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.  Cell. Signal.  2016  28:937-45  PubMed ID: 27132626   DOI: 10.1016/j.cellsig.2016.04.009
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708   DOI: 10.1186/s12885-016-2779-y
13760  Shoji T, Saito R, Chonan M, Shibahara I, Sato A, Kanamori M, Sonoda Y, Kondo T, Ishii N, Tominaga T.  Local convection-enhanced delivery of an anti-CD40 agonistic monoclonal antibody induces antitumor effects in mouse glioma models  Neuro Oncol  2016  18(8):1120-8  PubMed ID: 26917236   DOI: 10.1093/neuonc/now023
7557  Seki K, Kinashi Y, Takahashi S.  Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.  Anticancer Res.  2015  35:169-74  PubMed ID: 25550548  
4947  Matsuda Y, Ishiwata T, Yoshimura H, Hagio M, Arai T.  Inhibition of nestin suppresses stem cell phenotype of glioblastomas through the alteration of post-translational modification of heat shock protein HSPA8/HSC71  Cancer Lett  2015  357(2):602-1  PubMed ID: 25527454   DOI: 10.1016/j.canlet.2014.12.030
7738  Sunayama J, Sato A, Matsuda K, Tachibana K, Watanabe E, Seino S, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  FoxO3a functions as a key integrator of cellular signals that control glioblastoma stem-like cell differentiation and tumorigenicity.  Stem Cells  2011  29:1327-37  PubMed ID: 21793107   DOI: 10.1002/stem.696
7934  Matsuda Y, Kawamoto Y, Teduka K, Peng WX, Yamamoto T, Ishiwata T, Naito Z.  Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.  Int. J. Oncol.  2011  38:1253-8  PubMed ID: 21331444   DOI: 10.3892/ijo.2011.945
8024  Tahara K, Kato Y, Yamamoto H, Kreuter J, Kawashima Y.  Intracellular drug delivery using polysorbate 80-modified poly(D,L-lactide-co-glycolide) nanospheres to glioblastoma cells.  J Microencapsul  2011  28:29-36  PubMed ID: 21171814   DOI: 10.3109/02652048.2010.522258
16653  Liu Y, Liang Y, Zheng T, Yang G, Zhang X, Sun Z, Shi C, Zhao S.  Inhibition of heme oxygenase-1 enhances anti-cancer effects of arsenic trioxide on glioma cells  J Neurooncol  2011  104(2):449-58  PubMed ID: 21327864   DOI: 10.1007/s11060-010-0513-1
8111  Sunayama J, Sato A, Matsuda K, Tachibana K, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  Dual blocking of mTor and PI3K elicits a prodifferentiation effect on glioblastoma stem-like cells.  Neuro-oncology  2010  12:1205-19  PubMed ID: 20861085   DOI: 10.1093/neuonc/noq103
8338  Yamashita Y, Kasugai I, Sato M, Tanuma N, Sato I, Nomura M, Yamashita K, Sonoda Y, Kumabe T, Tominaga T, Katakura R, Shima H.  CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples.  J. Neurooncol.  2010  100:43-9  PubMed ID: 20217459   DOI: 10.1007/s11060-010-0147-3
8297  Sunayama J, Matsuda K, Sato A, Tachibana K, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  Crosstalk between the PI3K/mTOR and MEK/ERK pathways involved in the maintenance of self-renewal and tumorigenicity of glioblastoma stem-like cells.  Stem Cells  2010  28(11):1930-9  PubMed ID: 20857497   DOI: 10.1002/stem.521
14324  Gene expression profile changes correlating with radioresistance in human cell lines  Gene expression profile changes correlating with radioresistance in human cell lines  Int J Radiat Oncol Biol Phys  2006  65(1):234-4  PubMed ID: 16618578   DOI: 10.1016/j.ijrobp.2005.12.048


戻る 戻る 理研トップページへ