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Cell No. : Cell Name
RCB2530 : A172  update : 2020/08/27
CommentHuman cell line derived from glioblastoma. TKG0183 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Giard, D. J.
Year of deposit 2007
Original cell TKG0183
Animal human < Mammals
Genus Homo
Species sapiens
Tissue brain
Case history glioblastoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0183)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 20


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Reference
1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758

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User's Publication
11332  Toyama M, Hamaoka Y, Katoh H.  EphA3 is up-regulated by epidermal growth factor and promotes formation of glioblastoma cell aggregates.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30528229
11505  Kageyama-Yahara N, Yamamichi N, Takahashi Y, Takeuchi C, Matsumoto Y, Sakaguchi Y, Koike K.  Tandem repeats of the 5' flanking region of human MUC5AC have a role as a novel enhancer in MUC5AC gene expression.  Biochem Biophys Rep  2019  18:100632  PubMed ID: 30993217
11581  Teramoto K, Katoh H.  The cystine/glutamate antiporter xCT is a key regulator of EphA2 S897 phosphorylation under glucose-limited conditions.  Cell. Signal.  2019    PubMed ID: 31152846
11624  Furuse Y, Chaimongkol N, Okamoto M, Oshitani H.  Evolutionary and Functional Diversity of the 5' Untranslated Region of Enterovirus D68: Increased Activity of the Internal Ribosome Entry Site of Viral Strains during the 2010s.  Viruses  2019    PubMed ID: 31288421
11639  Taniguchi K, Ii H, Kageyama S, Takagi H, Chano T, Kawauchi A, Nakata S.  Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK-FOXO3a-p21 axis.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31345573
10522  Haryu S, Saito R, Jia W, Shoji T, Mano Y, Sato A, Kanamori M, Sonoda Y, Sampetrean O, Saya H, Tominaga T.  Convection-enhanced delivery of sulfasalazine prolongs survival in a glioma stem cell brain tumor model.  J. Neurooncol.  2018  136:23-31  PubMed ID: 28929335
10677  Yaekashiwa N, Otsuki S, Hayashi S, Kawase K.  Investigation of the non-thermal effects of exposing cells to 70-300 GHz irradiation using a widely tunable source.  J. Radiat. Res.  2018  59:116-121  PubMed ID: 29281029
4071  Zhiwei Qiao, Tadashi Kondo  Identification of cephalomannine as a drug candidate for glioblastoma via high-throughput drug screening  Journal of Electrophoresis  2018  62:17 
4125  Hamaoka Y, Negishi M, Katoh H.  Tyrosine kinase activity of EphA2 promotes its S897 phosphorylation and glioblastoma cell proliferation.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 29626472
9963  Kamemura K.  O-GlcNAc glycosylation stoichiometry of the FET protein family: only EWS is glycosylated with a high stoichiometry.  Biosci. Biotechnol. Biochem.  2017  81:541-546  PubMed ID: 27903134
10559  Goji T, Takahara K, Negishi M, Katoh H.  Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation.  J. Biol. Chem.  2017  292:19721-19732  PubMed ID: 29038291
7074  Hamaoka Y, Negishi M, Katoh H.  EphA2 is a key effector of the MEK/ERK/RSK pathway regulating glioblastoma cell proliferation.  Cell. Signal.  2016  28:937-45  PubMed ID: 27132626
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708
7557  Seki K, Kinashi Y, Takahashi S.  Influence of p53 status on the effects of boron neutron capture therapy in glioblastoma.  Anticancer Res.  2015  35:169-74  PubMed ID: 25550548
7738  Sunayama J, Sato A, Matsuda K, Tachibana K, Watanabe E, Seino S, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  FoxO3a functions as a key integrator of cellular signals that control glioblastoma stem-like cell differentiation and tumorigenicity.  Stem Cells  2011  29:1327-37  PubMed ID: 21793107
7934  Matsuda Y, Kawamoto Y, Teduka K, Peng WX, Yamamoto T, Ishiwata T, Naito Z.  Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.  Int. J. Oncol.  2011  38:1253-8  PubMed ID: 21331444
8024  Tahara K, Kato Y, Yamamoto H, Kreuter J, Kawashima Y.  Intracellular drug delivery using polysorbate 80-modified poly(D,L-lactide-co-glycolide) nanospheres to glioblastoma cells.  J Microencapsul  2010  28:29-36  PubMed ID: 21171814
8111  Sunayama J, Sato A, Matsuda K, Tachibana K, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  Dual blocking of mTor and PI3K elicits a prodifferentiation effect on glioblastoma stem-like cells.  Neuro-oncology  2010  12:1205-19  PubMed ID: 20861085
8338  Yamashita Y, Kasugai I, Sato M, Tanuma N, Sato I, Nomura M, Yamashita K, Sonoda Y, Kumabe T, Tominaga T, Katakura R, Shima H.  CDC25A mRNA levels significantly correlate with Ki-67 expression in human glioma samples.  J. Neurooncol.  2010  100:43-9  PubMed ID: 20217459
8297  Sunayama J, Matsuda K, Sato A, Tachibana K, Suzuki K, Narita Y, Shibui S, Sakurada K, Kayama T, Tomiyama A, Kitanaka C.  Crosstalk between the PI3K/mTOR and MEK/ERK pathways involved in the maintenance of self-renewal and tumorigenicity of glioblastoma stem-like cells.  Stem Cells  2010  28(11):1930-9  PubMed ID: 20857497



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