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Cell No. : Cell Name
RCB2403 : MG6  update : 2018/07/06
CommentMouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene. No significant production of infectious viral particles from MG6 cells was confirmed by bioassay using SC-1 cells.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) If any intellectual Property is generated during the use of the delivered Material, the Recipient shall consult with the Administrator of the Material at the NARO. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neurosci Lett 2006;407:205-210, Biochim Biophys Acta 2005;1726:177-186 ) designated by the DEPOSITOR is required.
approver's address
The National Agriculture and Food Research Organization
Institute of Agrobiological Sciences, Division of Animal Science
1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
Dr.Kitani, Hiroshi
Fax. +81-29-838-6043
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kitani, Hiroshi
Originator Kitani, Hiroshi
Year of deposit 2006
Another name MG6-1
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 1 day
Tissue brain
Classification transformed
Recombinant recombinant
Exogene human c-myc, neomycin, retroviral vector
Lifespan infinite
Morphology other (macrophage-like)
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml Insulin + 0.1mM 2-Mercaptoethanol
Antibiotics Free
Passage method pipetting
Coating dish Non-tissue culture dish
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Reference information Reference 9
User's Publication 43

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5301  Otani Y, Yamaguchi Y, Sato Y, Furuichi T, Ikenaka K, Kitani H, Baba H.  PLD$ is involved in phagocytosis of microglia: expression and localization changes of PLD4 are correlated with activation state of microglia.  PLoS One  2011  6(11):e27544  PubMed ID: 22102906
5300  Nakamichi K, Kitani H, Takayama-Ito M, Morimoto K, Kurane I, Saijo M.  Celastrol suppresses morphological and transcriptional responses in microglial cells upon stimulation with double-stranded RNA.  Int J Neurosci  2010  120(4):252-7  PubMed ID: 20374071
5299  Takenouchi T, Nakai M, Iwamaru Y, Sugama S, Tsukimoto M, Fujita M, Wei J, Sekigawa A, Sato M, Kojima S, Kitani H, Hashimoto M.  The activation of P2X7 receptor impairs lysosomal functions and stimulates the release of autophagolysosomes in microglial cells.  J Immunol  2009  182(4):2051-62  PubMed ID: 19201858
5298  Takenouchi T, Iwamaru Y, Sugama S, Sato M, Hashimoto M, Kitani H.  Lysophospholipids and ATP mutually suppress maturation and release of IL-1 beta in mouse microglial cells using a Rho-dependent pathway.  J Immunol  2008  180(12):7827-39  PubMed ID: 18523246
5295  Nakamichi K, Saiki M, Kitani H, Kuboyama Y, Morimoto K, Takayama-Ito M, Kurane I.  Roles of NF-kappaB and MAPK signaling pathways in morphological and cytoskeletal responses of microglia to double-stranded RNA.  Neurosci Lett  2007  414(3):222-7  PubMed ID: 17284350
5296  Takenouchi T, Sato M1, Kitani H1.  Lysophosphatidylcholine potentiates Ca2+ influx, pore formation and p44/42 MAP kinase phosphorylation mediated by P2X7 receptor activation in mouse microglial cells.  J Neurochem  2007  102(5):1518-1532  PubMed ID: 17437542
5297  Sugama S, Takenouchi T, Kitani H, Fujita M, Hashimoto M.  Activin as an anti-inflammatory cytokine produced by microglia.  J Neuroimmunol  2007  192(1-2):31-9  PubMed ID: 17976743
5293  Nakamichi K, Saiki M, Kitani H, Kuboyama Y, Morimoto K, Takayama-Ito M, Kurane I.  Suppressive effect of simvastatin on interferon-beta-induced expression of CC chemokine ligand 5 in microglia.  Neurosci Lett  2006  407(3):205-10  PubMed ID: 16978784
5294  Takenouchi T, Ogihara K, Sato M, Kitani H.  Inhibitory effects of U73122 and U73343 on Ca2+ influx and pore formation induced by the activation of P2X7 nucleotide receptors in mouse microglial cell line.  Biochim Biophys Acta  2005  1726(2):177-86  PubMed ID: 16122875

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User's Publication
11817  Suzuki T, Kohyama K, Moriyama K, Ozaki M, Hasegawa S, Ueno T, Saitoe M, Morio T, Hayashi M, Sakuma H.  Extracellular ADP augments microglial inflammasome and NF-κB activation via the P2Y12 receptor.  Eur. J. Immunol.  2020  50(2):205-219  PubMed ID: 31549730
12025  Ogawa K, Yagi T, Guo T, Takeda K, Ohguchi H, Koyama H, Aotani D, Imaeda K, Kataoka H, Tanaka T.  Pemafibrate, a selective PPARα modulator, and fenofibrate suppress microglial activation through distinct PPARα and SIRT1-dependent pathways.  Biochem. Biophys. Res. Commun.  2020    PubMed ID: 32005522
11351  Ni J, Wu Z, Stoka V, Meng J, Hayashi Y, Peters C, Qing H, Turk V, Nakanishi H.  Increased expression and altered subcellular distribution of cathepsin B in microglia induce cognitive impairment through oxidative stress and inflammatory response in mice.  Aging Cell  2019    PubMed ID: 30575263
5292  Huntula S, Saegusa H, Wang X, Zong S, Tanabe T.  Involvement of N-type Ca2+ channel in microglial activation and its implications to aging-induced exaggerated cytokine response.  Cell Calcium  2019  82:102059  PubMed ID: 31377554
5306  Saputra WD, Aoyama N, Komai M, Shirakawa H4,.  Menaquinone-4 Suppresses Lipopolysaccharide-Induced Inflammation in MG6 Mouse Microglia-Derived Cells by Inhibiting the NF-κB Signaling Pathway.  Int J Mol Sci  2019  20(9). pii: E2317  PubMed ID: 31083359
5308  Inoue T, Yamakage H, Tanaka M, Kusakabe T, Shimatsu A, Satoh-Asahara N.  Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.  Cells  2019  8(6):527  PubMed ID: 31159306
5316  Youssef M, Ibrahim A, Akashi K, Hossain MS.  PUFA-Plasmalogens Attenuate the LPS-Induced Nitric Oxide Production by Inhibiting the NF-kB, p38 MAPK and JNK Pathways in Microglial Cells.  Neuroscience  2019  15;397:18-30  PubMed ID: 30496826
5319  Fujikura M1, Iwahara N, Hisahara S, Kawamata J, Matsumura A, Yokokawa K, Saito T, Manabe T, Matsushita T, Suzuki S, Shimohama S.  CD14 and Toll-Like Receptor 4 Promote Fibrillar Aβ42 Uptake by Microglia Through A Clathrin-Mediated Pathway.  J Alzheimers Dis  2019  68(1):323-337  PubMed ID: 30775984
5320  Palikhe S, Ohashi W, Sakamoto T, Hattori K, Kawakami M, Andoh T, Yamazaki H, Hattori Y.  Regulatory Role of GRK2 in the TLR Signaling-Mediated iNOS Induction Pathway in Microglial Cells.  Front Pharmacol  2019  10:59  PubMed ID: 30778300
11614  Wang X, Saegusa H, Huntula S, Tanabe T.  Blockade of microglial Cav1.2 Ca2+ channel exacerbates the symptoms in a Parkinson's disease model.  Sci Rep  2019    PubMed ID: 31235768
11777  Ni J, Wu Z, Meng J, Saito T, Saido TC, Qing H, Nakanishi H.  An impaired intrinsic microglial clock system induces neuroinflammatory alterations in the early stage of amyloid precursor protein knock-in mouse brain.  J Neuroinflammation  2019  16(1):173  PubMed ID: 31470863
10687  Yasuhiro Nakagawa, Yuto Yano, Jeonggyu Lee, Yasutaka Anraku, Makoto Nakakido Makoto Nakakido, Kouhei Tsumoto, Horacio Cabral, and Mitsuhiro Ebara  Apoptotic Cell-Inspired Polymeric Particles for Controlling Microglial Inflammation toward Neurodegenerative Disease Treatment  ACS Biomater Sci Eng  2019  5:5705–5713 
10710  Kidana K, Tatebe T, Ito K, Hara N, Kakita A, Saito T, Takatori S, Ouchi Y, Ikeuchi T, Makino M, Saido TC, Akishita M, Iwatsubo T, Hori Y, Tomita T.  Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice.  EMBO Mol Med  2018    PubMed ID: 29311134
10775  Kawakami M, Hattori M, Ohashi W, Fujimori T, Hattori K, Takebe M, Tomita K, Yokoo H, Matsuda N, Yamazaki M, Hattori Y.  Role of G protein-coupled receptor kinase 2 in oxidative and nitrosative stress-related neurohistopathological changes in a mouse model of sepsis-associated encephalopathy.  J. Neurochem.  2018  145(6):474-488  PubMed ID: 29500815
11056  Meng J, Ni J, Wu Z, Jiang M, Zhu A, Qing H, Nakanishi H.  The Critical Role of IL-10 in the Anti-neuroinflammatory and Anti-oxidative Effects of Rheum tanguticum on Activated Microglia  Oxid Med Cell Longev  2018  2018:1083596  PubMed ID: 29854069
4423    Effects of antiepileptic drugs on microglial properties  Epilepsy & Seizure  2018  10, 22-32 
4293  Serizawa K, Tomizawa-Shinohara H, Magi M, Yogo K, Matsumoto Y.  Anti-IL-6 receptor antibody improves pain symptoms in mice with experimental autoimmune encephalomyelitis.  J. Neuroimmunol.  2018    PubMed ID: 29685293
4294  Shimamura M, Nakagami H, Shimizu H, Mukai H, Watanabe R, Okuzono T, Kawano T, Ikeda Y, Hayashi H, Yoshida S, Ju N, Mochizuki H, Morishita R.  Development of a novel RANKL-based peptide, microglial healing peptide1-AcN (MHP1-AcN), for treatment of ischemic stroke.  Sci Rep  2018    PubMed ID: 30538259
4295  Shimamura M, Nakagami H, Shimizu H, Wakayama K, Kawano T, Ikeda Y, Hayashi H, Yoshida S, Mochizuki H, Morishita R.  Therapeutic Effects of Systemic Administration of the Novel RANKL-Modified Peptide, MHP1, for Ischemic Stroke in Mice.  Biomed Res Int  2018    PubMed ID: 30151382
7030  Wu Z, Ni J, Liu Y, Teeling JL, Takayama F, Collcutt A, Ibbett P, Nakanishi H.  Cathepsin B plays a critical role in inducing Alzheimer's disease-like phenotypes following chronic systemic exposure to lipopolysaccharide from Porphyromonas gingivalis in mice.  Brain Behav. Immun.  2017  65:350-361  PubMed ID: 28610747
10102  Mahmoud ME, Fereig R, Nishikawa Y.  Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice.  Infect. Immun.  2017  85:  PubMed ID: 28138019
10138  Inoue T, Tanaka M, Masuda S, Ohue-Kitano R, Yamakage H, Muranaka K, Wada H, Kusakabe T, Shimatsu A, Hasegawa K, Satoh-Asahara N.  Omega-3 polyunsaturated fatty acids suppress the inflammatory responses of lipopolysaccharide-stimulated mouse microglia by activating SIRT1 pathways.  Biochim. Biophys. Acta  2017  1862:552-560  PubMed ID: 28254441
10527  Liu Y, Wu Z, Nakanishi Y, Ni J, Hayashi Y, Takayama F, Zhou Y, Kadawaki T, Nakanishi H.  Infection of microglia with Porphyromonas gingivalis promotes cell migration and an inflammatory response through the gingipain-mediated activation of protease-activated receptor-2 in mice.  Sci Rep  2017  7:11759  PubMed ID: 28924232
5311  Koshiguchi M, Komazaki H, Hirai S, Egashira Y.  Ferulic acid suppresses expression of tryptophan metabolic key enzyme indoleamine 2, 3-dioxygenase via NFκB and p38 MAPK in lipopolysaccharide-stimulated microglial cells.  Biosci Biotechnol Biochem  2017  81(5):966-971  PubMed ID: 28077028
9595  Hossain MS, Mineno K, Katafuchi T.  Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling.  PLoS ONE  2016  11:e0150846  PubMed ID: 26934370
9766  Takayama F, Hayashi Y, Wu Z, Liu Y, Nakanishi H.  Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system.  Sci Rep  2016  6:30006  PubMed ID: 27445174
9970  Kurinami H, Shimamura M, Nakagami H, Shimizu H, Koriyama H, Kawano T, Wakayama K, Mochizuki H, Rakugi H, Morishita R.  A Novel Therapeutic Peptide as a Partial Agonist of RANKL in Ischemic Stroke.  Sci Rep  2016  6:38062  PubMed ID: 27897273
7104  Hosain MZ, Mori T, Kishimura A, Katayama Y.  Synergy between phenotypic modulation and ROS neutralization in reduction of inflammatory response of hypoxic microglia by using phosphatidylserine and antioxidant containing liposomes.  J Biomater Sci Polym Ed  2015  27:290-302  PubMed ID: 26689775
7218  Ni J, Wu Z, Peterts C, Yamamoto K, Qing H, Nakanishi H.  The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage.  J. Neurosci.  2015  35:12488-501  PubMed ID: 26354916
7247  Takenouchi T, Tsukimoto M, Iwamaru Y, Sugama S, Sekiyama K, Sato M, Kojima S, Hashimoto M, Kitani H.  Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells.  Immunol. Lett.  2015  167:116-24  PubMed ID: 26277554
7369  Segawa S, Tatsumi N, Ohishi A, Nishida K, Nagasawa K.  Characterization of zinc uptake by mouse primary cultured astrocytes and microglia.  Metallomics  2015  7:1067-77  PubMed ID: 25924210
5303  Yu Z, Ono C, Aiba S, Kikuchi Y, Sora I, Matsuoka H, Tomita H.  Therapeutic concentration of lithium stimulates complement C3 production in dendritic cells and microglia via GSK-3 inhibition.  Glia  2015  63(2):257-70  PubMed ID: 25179772
5314  Bahrini I, Song JH, Diez D, Hanayama R.  Neuronal exosomes facilitate synaptic pruning by up-regulating complement factors in microglia.  Sci Rep  2015  5:7989  PubMed ID: 25612542
5313  Ishisaka A, Mukai R, Terao J, Shibata N, Kawai Y.  Specific localization of quercetin-3-O-glucuronide in human brain.  Arch Biochem Biophys  2014  557:11-7  PubMed ID: 24893148
5317  Jin Y, Sato K, Tobo A, Mogi C, Tobo M, Murata N, Ishii S, Im DS, Okajima F.  Inhibition of interleukin-1β production by extracellular acidification through the TDAG8/cAMP pathway in mouse microglia.  J Neurochem  2014  129(4):683-95  PubMed ID: 24447140
5321  Kotake M, Sato K, Mogi C, Tobo M, Aoki H, Ishizuka T, Sunaga N, Imai H, Kaira K, Hisada T, Yamada M, Okajima F.  Acidic pH increases cGMP accumulation through the OGR1/phospholipase C/Ca(2+)/neuronal NOS pathway in N1E-115 neuronal cells.  Cell Signal  2014  26(11):2326-32  PubMed ID: 25025574
5304  Liu Y, Wu Z, Zhang X, Ni J, Yu W, Zhou Y, Nakanishi H.  Leptomeningeal cells transduce peripheral macrophages inflammatory signal to microglia in reponse to Porphyromonas gingivalis LPS.  Mediators Inflamm  2013  2013:407562  PubMed ID: 24363500
5305  Wu Z, Zhu A, Takayama F, Okada R, Liu Y, Harada Y, Wu S, Nakanishi H.  Brazilian green propolis suppresses the hypoxia-induced neuroinflammatory responses by inhibiting NF-κB activation in microglia.  Oxid Med Cell Longev  2013  2013:906726  PubMed ID: 23983903
7591  Kitajima H, Komizu Y, Ueoka R.  Hybrid liposomes inhibit growth and invasion of human osteosarcoma cells leading to apoptosis.  Bioorg. Med. Chem. Lett.  2012  22:1784-7  PubMed ID: 22260774
7594  Yanagitai M, Itoh S, Kitagawa T, Takenouchi T, Kitani H, Satoh T.  Carnosic acid, a pro-electrophilic compound, inhibits LPS-induced activation of microglia.  Biochem. Biophys. Res. Commun.  2012  418:22-6  PubMed ID: 22214931
5312  Mukai R, Kawabata K, Otsuka S, Ishisaka A, Kawai Y, Ji ZS, Tsuboi H, Terao J.  Effect of quercetin and its glucuronide metabolite upon 6-hydroxydopamine-induced oxidative damage in Neuro-2a cells.  Free Radic Res  2012  46(8):1019-28  PubMed ID: 22404304
7884  Takenouchi T, Iwamaru Y, Sugama S, Tsukimoto M, Fujita M, Sekigawa A, Sekiyama K, Sato M, Kojima S, Conti B, Hashimoto M, Kitani H.  The activation of P2X7 receptor induces cathepsin D-dependent production of a 20-kDa form of IL-1β under acidic extracellular pH in LPS-primed microglial cells.  J. Neurochem.  2011  117:712-23  PubMed ID: 21395581
10347  Denehy TR, Choe JY, Gregori CA, Breen JL.  Modified Le Fort partial colpocleisis with Kelly urethral plication and posterior colpoperineoplasty in the medically compromised elderly: a comparison with vaginal hysterectomy, anterior colporrhaphy, and posterior colpoperineoplasty.  Am. J. Obstet. Gynecol.  1995  173:1697-701  PubMed ID: 8610747

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