Cell No. : Cell Name
RCB2400 : KT54
update : 2024/01/17
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| Comment | Hybridoma producing monoclonal antibody against an antigen of C. elegans embryo. Antigen is not determined but that on centrosome and P-granule. |
| Comment from the depositor | |
| Terms and conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Sugimoto, Asako:RIKEN Center for Developmental Biology) is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Sugimoto, Asako
|
| Originator |
Takeda, Kazumasa
|
| Year of deposit |
2006
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_X677
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|
hybridoma
|
Fusion_cell_1 name |
spleen cell
|
| Fusion_cell_1 strain |
mouse, BALB/c
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| Fusion_cell_2 name |
P3-X63-Ag8.653
|
| Fusion_cell_2 strain |
mouse
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|
Fusion method |
PEG, HAT selection
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|
Antibody name |
KT54
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| Subclass |
IgM(Kappa)
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| Immunogen |
C. elegans embryo
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|
Antigen_positive |
Centrosome, p-granule (C. elegans embryo)
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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RPMI1640 + 15% FBS + 1mM Sodium Pyruvate + 5% Briclone + 55μM 2-Mercaptoethanol
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| Antibiotics |
|
Free
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Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 6 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Antibody subclass |
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IgM(Kappa)
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
0
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