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Cell No. : Cell Name
RCB2392 : KT29  update : 2021/09/07
CommentHybridoma producing monoclonal antibody against an antigen of C. elegans embryo. Antigen is not determined but that on egg shell.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR (Sugimoto, Asako:RIKEN Center for Developmental Biology) is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Sugimoto, Asako
Originator Takeda, Kazumasa
Year of deposit 2006
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_X669
hybridoma Fusion_cell_1 name spleen cell
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name P3-X63-Ag8.653
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name KT29
Subclass IgM(Kappa)
Immunogen C. elegans embryo
Antigen_positive Egg shell (C. elegans embryo)
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS + 1mM Sodium Pyruvate + 10% Briclone + 55μM 2-Mercaptoethanol
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgM(Kappa)
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lot info
Reference information Reference 0
User's Publication 0


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