Cell No. : Cell Name
RCB2366 : Hu5/E18 update : 2024/08/20 |
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Comment | Human multipotent stem cell line able to differentiate into muscle, bone, and adipocytes. | |||
Comment from the depositor | ||||
Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2006;348:1383-1388, Mech Dev 2008;125:257-269) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. | |||
Remarks | ||||
Order Form | Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Hashimoto, Naohiro | ||
Originator | Hashimoto, Naohiro | |||
Year of deposit | 2006 | |||
Another name | E18 | |||
Original cell | Hu5/E | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Race | Japanese | |||
Gender | Female | |||
Age at sampling | 42 years | |||
Tissue | subcutaneous muscle | |||
Classification | semi-normal | |||
Recombinant | recombinant | |||
Exogene | hTERT, HPV16-E7 | Lifespan | infinite | |
Morphology | fibroblast-like | |||
Cellosaurus(Expasy) | CVCL_U895 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | DMEM (high glucose) + 20% FBS + 2% Ultroser G | |||
Antibiotics | Free | Passage method | 0.25% Trypsin | |
Coating dish | type I collagen coated dish | Culture information | Passage cell No | 2-4 x 10 5 cells/100 mm dish |
SC frequency | Subculture : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 10 % | |||
Freeze medium | 90% FBS + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma/Acholeplasma | (-) | |||
Animal PCR | OK | |||
Isozyme | LD, NP | |||
STR(human) | OK | |||
Images | ||||
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Reference information | Reference | 4 | ||
User's Publication | 2 | |||