Cell No. : Cell Name
RCB2366 : Hu5/E18
update : 2026/04/20
|
| Comment | Human multipotent stem cell line able to differentiate into muscle, bone, and adipocytes. |
| Comment from the depositor | |
| Terms and conditions | 1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2006;348:1383-1388, Mech Dev 2008;125：257-269) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Hashimoto, Naohiro
|
| Originator |
Hashimoto, Naohiro
|
| Year of deposit |
2006
|
| Another name |
E18
|
| Original cell |
Hu5/E
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Female
|
| Age at sampling |
42 years
|
| Tissue |
subcutaneous muscle
|
| Classification |
semi-normal
|
| Recombinant |
recombinant
|
| Exogene |
hTERT, HPV16-E7
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| SDS |
SDS_DMSO(Japanese) 
|
| Cellosaurus(Expasy) |
CVCL_U895
|
|
| |
deposit info |
lot info |
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Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (high glucose) + 20% FBS + 2% Ultroser G
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
| Coating dish |
|
type I collagen coated dish
|
Culture information
|
Passage cell No |
|
2-4 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
10
%
|
| Freeze medium |
|
90% FBS + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
3
|
| Reference |
|
15666
Shiomi K, Kiyono T, Okamura K, Uezumi M, Goto Y, Yasumoto S, Shimizu S, Hashimoto N.
CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential
Gene Ther
2011
18(9):857-66
PubMed ID: 21490680
DOI: 10.1038/gt.2011.44
|
|
5696
Hashimoto N, Kiyono T, Wada MR, Umeda R, Goto Y, Nonaka I, Shimizu S, Yasumoto S, Inagawa-Ogashiwa M.
Osteogenic properties of human myogenic progenitor cells.
Mech Dev
2008
125(3-4):257-69
PubMed ID: 18164186
DOI: 10.1016/j.mod.2007.11.004
|
|
5692
Hashimoto N, Kiyono T, Wada MR, Shimizu S, Yasumoto S, Inagawa M.
Immortalization of human myogenic progenitor cell clone retaining multipotentiality.
Biochem Biophys Res Commun
2006
348(4):1383-8
PubMed ID: 16919240
DOI: 10.1016/j.bbrc.2006.08.006
|
|
15658
Wada MR, Inagawa-Ogashiwa M, Shimizu S, Yasumoto S, Hashimoto N.
Generation of different fates from multipotent muscle stem cells
Development
2002
129(12):2987-95
PubMed ID: 12050145
DOI: 10.1242/dev.129.12.2987
|
| User's Publication |
|
23685
Zhou L, Feng C, Lin J, Geng M, Qu D, Xing J, Lin H, Ma X, Nakanishi R, Maeshige N, Kondo H, Fujino H.
Enhancement of Hypoxia-Induced Autophagy via the HIF-1apha/BNIP3 Pathway Promotes Proliferation and Myogenic Differentiation of Aged Skeletal Muscle Satellite Cells.
Life (Basel)
2026
16(1)
PubMed ID: 41598299
DOI: 10.3390/life16010144
|
|
5693
Goto K, Imamura K, Komatsu K3, Mitani K, Aiba K, Nakatsuji N, Inoue M, Kawata A, Yamashita H, Takahashi R, Inoue H.
Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors.
Mol Ther Methods Clin Dev
2017
4:115-125
PubMed ID: 28344997
DOI: 10.1016/j.omtm.2016.12.007
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5694
Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.
Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.
PLoS One
2013
8(4):e61540
PubMed ID: 23626698
DOI: 10.1371/journal.pone.0061540
|
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