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Cell No. : Cell Name
RCB2366 : Hu5/E18  update : 2022/01/21
CommentHuman multipotent stem cell line able to differentiate into muscle, bone, and adipocytes.
Comment from the depositor
Terms and conditions1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2006;348:1383-1388, Mech Dev 2008;125:257-269) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hashimoto, Naohiro
Originator Hashimoto, Naohiro
Year of deposit 2006
Another name E18
Original cell Hu5/E
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 42 years
Tissue subcutaneous muscle
Classification semi-normal
Recombinant recombinant
Exogene hTERT, HPV16-E7
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_U895
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 20% FBS + 2% Ultroser G
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish type I collagen coated dish
Culture information Passage cell No 2-4 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 10 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 2


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Reference
15666  Shiomi K, Kiyono T, Okamura K, Uezumi M, Goto Y, Yasumoto S, Shimizu S, Hashimoto N.  CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential  Gene Ther  2011  18(9):857-66  PubMed ID: 21490680   DOI: 10.1038/gt.2011.44
5696  Hashimoto N, Kiyono T, Wada MR, Umeda R, Goto Y, Nonaka I, Shimizu S, Yasumoto S, Inagawa-Ogashiwa M.  Osteogenic properties of human myogenic progenitor cells.  Mech Dev  2008  125(3-4):257-69  PubMed ID: 18164186   DOI: 10.1016/j.mod.2007.11.004
5692  Hashimoto N, Kiyono T, Wada MR, Shimizu S, Yasumoto S, Inagawa M.  Immortalization of human myogenic progenitor cell clone retaining multipotentiality.  Biochem Biophys Res Commun  2006  348(4):1383-8  PubMed ID: 16919240   DOI: 10.1016/j.bbrc.2006.08.006
15658  Wada MR, Inagawa-Ogashiwa M, Shimizu S, Yasumoto S, Hashimoto N.  Generation of different fates from multipotent muscle stem cells  Development  2002  129(12):2987-95  PubMed ID: 12050145   DOI: 10.1242/dev.129.12.2987

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User's Publication
5693  Goto K, Imamura K, Komatsu K3, Mitani K, Aiba K, Nakatsuji N, Inoue M, Kawata A, Yamashita H, Takahashi R, Inoue H.  Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors.  Mol Ther Methods Clin Dev  2017  4:115-125  PubMed ID: 28344997   DOI: 10.1016/j.omtm.2016.12.007
5694  Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.  Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.  PLoS One  2013  8(4):e61540  PubMed ID: 23626698   DOI: 10.1371/journal.pone.0061540



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