Cell No. : Cell Name
							 RCB2365 : JHONA
						
						 update : 2023/10/16
						 
						 
					 
						
						
						
							
								Comment Human cell line derived from ovarian squamous cell carcinoma. Application consideration  
							 
							
								Comment from the depositor  
							 
							
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										    			Contact Us cellbank.brc@riken.jp RIKEN, BioResource Research Center Cell Engineering Division
										    			
													   	 Dr.ISHIKAWA Hiroshi (Cell Bank)
													 	
													 	
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								Basic information
							
							
							 
						
						
						
							
								
									
										
										
										
										
				
                                
                                
                                
                                        Depositor 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Ishikawa, Hiroshi
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Originator 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Ishikawa, H. & Kimura, E.
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Year of deposit 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								2002
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Animal 
                                
                                
				
				
				
					
						
						
							
								 
								
								 	_human
								
								
								
							
								 
								
								
								 	
								 		< Mammals
								 	
								
								
							
						
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Genus 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Homo
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Species 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								sapiens
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Race 
                                
                                
				
				
				
					
						
						
						
							Japanese
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Gender 
                                
                                
				
				
				
					
						
						
						
							Female
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Age at sampling 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								67 years
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Tissue 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								ovary
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Metastatic ability 
                                
                                
				
				
				
					
						
						
						
							Yes
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Metastatic tissue 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								lymph node
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Classification 
                                
                                
				
				
				
					
						
						
						
							cancer
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Year of origin 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								2001
							
							
						
						
							
						
					 
				
				
					 
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							
							
							
							
								
							
							
							 
						
						
						
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Lifespan 
                                
                                
				
				
				
					
						
						
						
							infinite
						
						
						
						
							
						
					 
				
				
					
					
						
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Morphology 
                                
                                
				
				
				
					
						
						
						
							epithelial-like
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Cellosaurus(Expasy) 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								CVCL_4646 
							
							
						
						
							
						
					 
				
				
					 
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								
									
										
										
											
											
											  
											deposit info  
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												Medium 
												Medium List  
											 
										
									
								
						
						
						
						
						
					
						
						
						
						
							
							
							
							
								
							
							
							 
						
						
						
							
								
									
									
										
										
										
										
				
				
				
				
					Culture type 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							Adherent cells
						
						
						
						
							
						
					 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Culture medium 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								DMEM/HamF12 + 20% FBS + 0.1mM NEAA
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Antibiotics 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								Free
							
							
						
						
							
						
					 
				
				
					 
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							
							
							
							
								
							
							
							 
						
						
						
							
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Passage method 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								0.25% Trypsin
							
							
						
						
							
						
					 
				
				
					
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							
							
							
							
								Culture information
							
							
							 
						
						
						
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Passage ratio 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								1 : 8 split
							
							
						
						
							
						
					 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					SC frequency 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								Subculture : once/2 weeks, Medium Renewal : 2 times/week
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Temperature 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								37
							
							
						
						
							℃
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					CO2 concentration 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								5
							
							
						
						
							%
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Freeze medium 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								Medium + 10% DMSO
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Freezing method 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							Slow freezing
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Mycoplasma/Acholeplasma 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							(-)
						
						
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Isozyme 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							
						
						
						
							
							
							
								LD, NP
							
							
						
						
							
						
					 
				
				
					 
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					STR(human) 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 
					
					
						
						
						
							OK
						
						
						
						
							
						
					 
				
				
					 
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
						
							
							
							
								Images 
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								Reference information 
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