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Cell No. : Cell Name
RCB2364 : HS-ES-1  update : 2022/08/18
CommentHuman cell line derived from epithelioid sarcoma. RCB2360 and RCB2361 were derived from another different patient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sonobe, Hiroshi
Originator Sonobe, Hiroshi
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Tissue unknown
Disease name epithelioid sarcoma, recurrence
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8713
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 0
User's Publication 2

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User's Publication
20775  Rasmussen SV, Jin JX, Bickford LR, Woods AD, Sahm F, Crawford KA, Nagamori K, Goto H, Torres KE, Sidoni A, Rudzinski ER, Thway K, Jones RL, Ciulli A, Wright H, Lathara M, Srinivasa G, Kannan K, Huang PH, Grünewald TGP, Berlow NE, Keller C.  Functional genomic analysis of epithelioid sarcoma reveals distinct proximal and distal subtype biology.  Clin Transl Med  2022  12(7):e961  PubMed ID: 35839307   DOI: 10.1002/ctm2.961
13518  Nakayama RT, Pulice JL, Valencia AM, McBride MJ, McKenzie ZM, Gillespie MA, Ku WL, Teng M, Cui K, Williams RT, Cassel SH, Qing H, Widmer CJ, Demetri GD, Irizarry RA, Zhao K, Ranish JA, Kadoch C.  SMARCB1 is required for widespread BAF complex-mediated activation of enhancers and bivalent promoters  Nat Genet  2017  49(11):1613-1623  PubMed ID: 28945250   DOI: 10.1038/ng.3958

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