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Cell No. : Cell Name
RCB2358 : HLtat  update : 2021/05/20
CommentA subline of the HeLa cells. HeLa cells contained stably integrated copies of the HIV-1 LTR promoter linked to a synthetic tat gene.
Comment from the depositor
Terms and conditionsRequired written permission from Dr. Barbara K. Felber or Dr. George N. Pavlakis..Acknowledgment for publications should read ”The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HLtat from Dr. Barbara K. Felber and Dr. George N.” Also include the reference (J Virol 64, 3734-3741, 1990, J Virol 64, 2519-2529,1990.)
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Barbara K. Felber & George N. Pavlakis
Year of deposit 2006
Original cell HeLa
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene tat gene, neomycin resistance,
History NIH AIDS Research & Reference Reagent Program
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_8164
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 0


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Reference
3618  Felber BK, Drysdale CM, Pavlakis GN.  Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein.  J Virol  1990  64(8):3734-41  PubMed ID: 2196381  
3619  Schwartz S, Felber BK, Benko DM, Fenyö EM, Pavlakis GN.  Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1.  J Virol  1990  64(6):2519-29  PubMed ID: 2335812  
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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