Cell No. : Cell Name
RCB2358 : HLtat
update : 2023/10/16
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| Comment | A subline of the HeLa cells. HeLa cells contained stably integrated copies of the HIV-1 LTR promoter linked to a synthetic tat gene. |
| Comment from the depositor | |
| Terms and conditions | Required written permission from Dr. Barbara K. Felber or Dr. George N. Pavlakis..Acknowledgment for publications should read ”The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HLtat from Dr. Barbara K. Felber and Dr. George N.” Also include the reference (J Virol 64, 3734-3741, 1990, J Virol 64, 2519-2529,1990.) |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Barbara K. Felber & George N. Pavlakis
|
| Year of deposit |
2006
|
| Original cell |
HeLa
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
tat gene, neomycin resistance,
|
| History |
NIH AIDS Research & Reference Reagent Program
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_8164
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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|
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
3618
Felber BK, Drysdale CM, Pavlakis GN.
Feedback regulation of human immunodeficiency virus type 1 expression by the Rev protein.
J Virol
1990
64(8):3734-41
PubMed ID: 2196381
DOI: 10.1128/JVI.64.8.3734-3741.1990
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|
3619
Schwartz S, Felber BK, Benko DM, Fenyö EM, Pavlakis GN.
Cloning and functional analysis of multiply spliced mRNA species of human immunodeficiency virus type 1.
J Virol
1990
64(6):2519-29
PubMed ID: 2335812
DOI: 10.1128/JVI.64.6.2519-2529.1990
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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