Cell No. : Cell Name
RCB2356 : HeLa-CD4-LTR-β-gal
update : 2023/10/16
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| Comment | A subline of the HeLa cells. Expressing human CD4 and Neomycin-resistant gene. One copy of the HIV-1 LTR (nt-138 to +80) linked to the β-galactosidase gene is integrated. |
| Comment from the depositor | |
| Terms and conditions | Acknowledgment for publications should read “The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HeLa-CD4-LTR- β-gal from Dr. Michael Emerman.“ Also include the reference (J Virol 66, 2232-2239, 1992.) |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Michael Emerman
|
| Year of deposit |
2006
|
| Original cell |
HeLa
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
human CD4, neomycin, hygromycin resistance, β-galactosidase, SV40 nulear localizing sequence
|
| History |
NIH AIDS Research & Reference Reagent Program
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_D259
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
5184
Somia NV1, Miyoshi H, Schmitt MJ, Verma IM.
Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.
J Virol
2000
74(9):4420-4
PubMed ID: 10756057
DOI: 10.1128/jvi.74.9.4420-4424.2000
|
|
5189
Lin PF, Samanta H, Bechtold CM, Deminie CA, Patick AK, Alam M, Riccardi K, Rose RE, White RJ, Colonno RJ.
Characterization of siamycin I, a human immunodeficiency virus fusion inhibitor.
Antimicrob Agents Chemother
1996
40(1):133-8
PubMed ID: 8787894
DOI: 10.1128/AAC.40.1.133
|
|
5187
Kimpton J, Emerman M.
Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.
J Virol
1992
66(4):2232-9
PubMed ID: 1548759
DOI: 10.1128/JVI.66.4.2232-2239.1992
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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