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Cell No. : Cell Name
RCB2356 : HeLa-CD4-LTR-β-gal  update : 2023/10/16
CommentA subline of the HeLa cells. Expressing human CD4 and Neomycin-resistant gene. One copy of the HIV-1 LTR (nt-138 to +80) linked to the β-galactosidase gene is integrated.
Comment from the depositor
Terms and conditionsAcknowledgment for publications should read “The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HeLa-CD4-LTR- β-gal from Dr. Michael Emerman.“ Also include the reference (J Virol 66, 2232-2239, 1992.)
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Miyoshi, Hiroyuki
Originator Michael Emerman
Year of deposit 2006
Original cell HeLa
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene human CD4, neomycin, hygromycin resistance, β-galactosidase, SV40 nulear localizing sequence
History NIH AIDS Research & Reference Reagent Program
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_D259
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 4
User's Publication 0

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5184  Somia NV1, Miyoshi H, Schmitt MJ, Verma IM.  Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.  J Virol  2000  74(9):4420-4  PubMed ID: 10756057   DOI: 10.1128/jvi.74.9.4420-4424.2000
5189  Lin PF, Samanta H, Bechtold CM, Deminie CA, Patick AK, Alam M, Riccardi K, Rose RE, White RJ, Colonno RJ.  Characterization of siamycin I, a human immunodeficiency virus fusion inhibitor.  Antimicrob Agents Chemother  1996  40(1):133-8  PubMed ID: 8787894   DOI: 10.1128/AAC.40.1.133
5187  Kimpton J, Emerman M.  Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene.  J Virol  1992  66(4):2232-9  PubMed ID: 1548759   DOI: 10.1128/JVI.66.4.2232-2239.1992
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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