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Cell No. : Cell Name
RCB2355 : HeLa CD4+Clone1022  update : 2021/05/20
CommentA subline of the HeLa cells. Expressing human CD4 and Neomycin-resistant gene.
Comment from the depositor
Terms and conditionsAcknowledgment for publications should read "The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HeLa CD4 Clone 1022 from Dr. Bruce Chesebro." Also include the reference (J Infect Dis 163, 64-70, 1991.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Bruce Chesebro
Year of deposit 2006
Original cell HeLa
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 31 years
Tissue cervix
Disease name cervical carcinoma
Classification cancer
Recombinant recombinant
Exogene human CD4 gene, neomycin resistance gene
History NIH AIDS Research & Reference Reagent Program
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_U339
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
5189  Lin PF, Samanta H, Bechtold CM, Deminie CA, Patick AK, Alam M, Riccardi K, Rose RE, White RJ, Colonno RJ.  Characterization of siamycin I, a human immunodeficiency virus fusion inhibitor.  Antimicrob Agents Chemother  1996  40(1):133-8  PubMed ID: 8787894
5185  Chesebro B1, Wehrly K, Metcalf J, Griffin DE.  Use of a new CD4-positive HeLa cell clone for direct quantitation of infectious human immunodeficiency virus from blood cells of AIDS patients.  J Infect Dis  1991  163(1):64-70  PubMed ID: 1984477
5186  Chesebro B, Nishio J, Perryman S, Cann A, O'Brien W, Chen IS, Wehrly K.  Identification of human immunodeficiency virus envelope gene sequences influencing viral entry into CD4-positive HeLa cells, T-leukemia cells, and macrophages.  J Virol  1991  65(11):5782-9  PubMed ID: 1920616
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

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