Cell No. : Cell Name
RCB2355 : HeLa CD4+Clone1022
update : 2023/10/16
|
| Comment | A subline of the HeLa cells. Expressing human CD4 and Neomycin-resistant gene. |
| Comment from the depositor | |
| Terms and conditions | Acknowledgment for publications should read "The following reagent was obtained though the AIDS Research & Reference Reagent Program. Division of AIDS, NIAID, NIH:HeLa CD4 Clone 1022 from Dr. Bruce Chesebro." Also include the reference (J Infect Dis 163, 64-70, 1991. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Bruce Chesebro
|
| Year of deposit |
2006
|
| Original cell |
HeLa
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
31 years
|
| Tissue |
cervix
|
| Disease name |
cervical carcinoma
|
| Classification |
cancer
|
| Recombinant |
recombinant
|
| Exogene |
human CD4 gene, neomycin resistance gene
|
| History |
NIH AIDS Research & Reference Reagent Program
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_U339
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
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|
| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
5189
Lin PF, Samanta H, Bechtold CM, Deminie CA, Patick AK, Alam M, Riccardi K, Rose RE, White RJ, Colonno RJ.
Characterization of siamycin I, a human immunodeficiency virus fusion inhibitor.
Antimicrob Agents Chemother
1996
40(1):133-8
PubMed ID: 8787894
DOI: 10.1128/AAC.40.1.133
|
|
5185
Chesebro B1, Wehrly K, Metcalf J, Griffin DE.
Use of a new CD4-positive HeLa cell clone for direct quantitation of infectious human immunodeficiency virus from blood cells of AIDS patients.
J Infect Dis
1991
163(1):64-70
PubMed ID: 1984477
DOI: 10.1093/infdis/163.1.64
|
|
5186
Chesebro B, Nishio J, Perryman S, Cann A, O'Brien W, Chen IS, Wehrly K.
Identification of human immunodeficiency virus envelope gene sequences influencing viral entry into CD4-positive HeLa cells, T-leukemia cells, and macrophages.
J Virol
1991
65(11):5782-9
PubMed ID: 1920616
DOI: 10.1128/JVI.65.11.5782-5789.1991
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
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