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Cell No. : Cell Name
RCB2354 : 293gp  update : 2024/06/12
CommentA subline of 293. Expressing MoMLV (Moloney murine leukemia virus) gag/pol. A retroviral vector packaging cell line.
Comment from the depositor
Terms and conditions1) Can be used only by not-for-profit entities and only for not-for-profit academic purposes. 2) In publishing the research results to be obtained by use of 293gp, an acknowledgment to Dr. N.V. Somia is requested.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Miyoshi, Hiroyuki
Year of deposit 2006
Original cell HEK293
Animal _human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue kidney
Classification transformed
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E072
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 9

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5184  Somia NV1, Miyoshi H, Schmitt MJ, Verma IM.  Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.  J Virol  2000  74(9):4420-4  PubMed ID: 10756057   DOI: 10.1128/jvi.74.9.4420-4424.2000

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User's Publication
19752  Ichikawa T, Suekane A, Nakahata S, Iha H, Shimoda K, Murakami T, Morishita K.  Inhibition of PRMT5/MEP50 Arginine Methyltransferase Activity Causes Cancer Vulnerability in NDRG2low Adult T-Cell Leukemia/Lymphoma  Int J Mol Sci  2024  25(5):2842  PubMed ID: 38474089   DOI: 10.3390/ijms25052842
21686  Deborah EA, Nabekura T, Shibuya K, Shibuya A.  THEMIS2 Impairs Antitumor Activity of NK Cells by Suppressing Activating NK Receptor Signaling.  J Immunol  2024    PubMed ID: 38619282   DOI: 10.4049/jimmunol.2300771
19632  ukasawa K, Lyu J, Kubo T, Tanaka Y, Suzuki A, Horie T, Tomizawa A, Osumi R, Iwahashi S, Tokumura K, Murata M, Kobayashi M, Todo T, Hirao A, Hinoi E.  MEK5-ERK5 Axis Promotes Self-renewal and Tumorigenicity of Glioma Stem Cells  Cancer Res Commun  2023  3(1):148-159  PubMed ID: 36968222   DOI: 10.1158/2767-9764.CRC-22-0243
21466  Nabekura T, Deborah EA, Tahara S, Arai Y, Love PE, Kako K, Fukamizu A, Muratani M, Shibuya A.  Themis2 regulates natural killer cell memory function and formation.  Nat Commun  2023  14(1):7200  PubMed ID: 37938555   DOI: 10.1038/s41467-023-42578-8
20631  Yamashita S, Hashimoto K, Sawada I, Ogawa M, Nakatsuka E, Kawano M, Kinose Y, Kodama M, Sawada K, Kimura T.  Endometrial galectin-3 causes endometriosis by supporting eutopic endometrial cell survival and engraftment in the peritoneal cavity.  Am J Reprod Immunol  2022    PubMed ID: 35366371   DOI: 10.1111/aji.13533
20774  Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.  Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.  Nat Commun  2022  13(1):4063  PubMed ID: 35831322   DOI: 10.1038/s41467-022-31690-w
20954  Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M.  ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.  Nat Commun  2022  13(1):7476  PubMed ID: 36463234   DOI: 10.1038/s41467-022-35230-4
9849  Kanehira M, Fujiwara T, Nakajima S, Okitsu Y, Onishi Y, Fukuhara N, Ichinohasama R, Okada Y, Harigae H.  An Lysophosphatidic Acid Receptors 1 and 3 Axis Governs Cellular Senescence of Mesenchymal Stromal Cells and Promotes Growth and Vascularization of Multiple Myeloma.  Stem Cells  2017  35:739-753  PubMed ID: 27641212   DOI: 10.1002/stem.2499
7274  Tohmonda T, Yoda M, Iwawaki T, Matsumoto M, Nakamura M, Mikoshiba K, Toyama Y, Horiuchi K.  IRE1α/XBP1-mediated branch of the unfolded protein response regulates osteoclastogenesis.  J. Clin. Invest.  2015  125:3269-79  PubMed ID: 26193638   DOI: 10.1172/JCI76765

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