Cell No. : Cell Name
RCB2354 : 293gp
update : 2022/12/21
|
| Comment | A subline of 293. Expressing MoMLV (Moloney murine leukemia virus) gag/pol. A retroviral vector packaging cell line. |
| Comment from the depositor | |
| Terms and conditions | 1) Can be used only by not-for-profit entities and only for not-for-profit academic purposes. 2) In publishing the research results to be obtained by use of 293gp, an acknowledgment to Dr. N.V. Somia is requested. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Year of deposit |
2006
|
| Original cell |
HEK293
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Age at sampling |
embryo
|
| Tissue |
kidney
|
| Classification |
transformed
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_E072
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
1
|
| User's Publication |
5
|
| User's Publication |
|
20631
Yamashita S, Hashimoto K, Sawada I, Ogawa M, Nakatsuka E, Kawano M, Kinose Y, Kodama M, Sawada K, Kimura T.
Endometrial galectin-3 causes endometriosis by supporting eutopic endometrial cell survival and engraftment in the peritoneal cavity.
Am J Reprod Immunol
2022
PubMed ID: 35366371
DOI: 10.1111/aji.13533
|
|
20774
Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.
Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.
Nat Commun
2022
13(1):4063
PubMed ID: 35831322
DOI: 10.1038/s41467-022-31690-w
|
|
20954
Ohe S, Kubota Y, Yamaguchi K, Takagi Y, Nashimoto J, Kozuka-Hata H, Oyama M, Furukawa Y, Takekawa M.
ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II.
Nat Commun
2022
13(1):7476
PubMed ID: 36463234
DOI: 10.1038/s41467-022-35230-4
|
|
9849
Kanehira M, Fujiwara T, Nakajima S, Okitsu Y, Onishi Y, Fukuhara N, Ichinohasama R, Okada Y, Harigae H.
An Lysophosphatidic Acid Receptors 1 and 3 Axis Governs Cellular Senescence of Mesenchymal Stromal Cells and Promotes Growth and Vascularization of Multiple Myeloma.
Stem Cells
2017
35:739-753
PubMed ID: 27641212
DOI: 10.1002/stem.2499
|
|
7274
Tohmonda T, Yoda M, Iwawaki T, Matsumoto M, Nakamura M, Mikoshiba K, Toyama Y, Horiuchi K.
IRE1α/XBP1-mediated branch of the unfolded protein response regulates osteoclastogenesis.
J. Clin. Invest.
2015
125:3269-79
PubMed ID: 26193638
DOI: 10.1172/JCI76765
|
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