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Cell No. : Cell Name
RCB2354 : 293gp  update : 2022/08/24
CommentA subline of 293. Expressing MoMLV (Moloney murine leukemia virus) gag/pol. A retroviral vector packaging cell line.
Comment from the depositor
Terms and conditions1) Can be used only by not-for-profit entities and only for not-for-profit academic purposes. 2) In publishing the research results to be obtained by use of 293gp, an acknowledgment to Dr. N.V. Somia is requested.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Year of deposit 2006
Original cell HEK293
Animal human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue kidney
Classification transformed
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E072
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 4


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Reference
5184  Somia NV1, Miyoshi H, Schmitt MJ, Verma IM.  Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.  J Virol  2000  74(9):4420-4  PubMed ID: 10756057   DOI: 10.1128/jvi.74.9.4420-4424.2000

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User's Publication
20631  Yamashita S, Hashimoto K, Sawada I, Ogawa M, Nakatsuka E, Kawano M, Kinose Y, Kodama M, Sawada K, Kimura T.  Endometrial galectin-3 causes endometriosis by supporting eutopic endometrial cell survival and engraftment in the peritoneal cavity.  Am J Reprod Immunol  2022    PubMed ID: 35366371   DOI: 10.1111/aji.13533
20774  Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.  Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.  Nat Commun  2022  13(1):4063  PubMed ID: 35831322   DOI: 10.1038/s41467-022-31690-w
9849  Kanehira M, Fujiwara T, Nakajima S, Okitsu Y, Onishi Y, Fukuhara N, Ichinohasama R, Okada Y, Harigae H.  An Lysophosphatidic Acid Receptors 1 and 3 Axis Governs Cellular Senescence of Mesenchymal Stromal Cells and Promotes Growth and Vascularization of Multiple Myeloma.  Stem Cells  2017  35:739-753  PubMed ID: 27641212   DOI: 10.1002/stem.2499
7274  Tohmonda T, Yoda M, Iwawaki T, Matsumoto M, Nakamura M, Mikoshiba K, Toyama Y, Horiuchi K.  IRE1α/XBP1-mediated branch of the unfolded protein response regulates osteoclastogenesis.  J. Clin. Invest.  2015  125:3269-79  PubMed ID: 26193638   DOI: 10.1172/JCI76765



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