Cell No. : Cell Name
RCB2352 : 633 update : 2023/10/16 |
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Comment | Human lung cancer cell line expressing capsid protein of human adenovirus. | |||
Comment from the depositor | ||||
Terms and conditions | 1) The BIOLOGICAL RESOURCE is provided as a service to research cpmmunity.2) A permission of the approver (Dr.Glen Nemerow & Scripps Research Institute) is required. | |||
Remarks | ||||
approver's address |
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Order Form | Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)   | |||
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
Basic information | Depositor | Yokoyama, K. & Nakamura, Y. | ||
Originator | Nemerow, Glen | |||
Year of deposit | 2006 | |||
Original cell | A549 | |||
Animal | _human < Mammals | |||
Genus | Homo | |||
Species | sapiens | |||
Gender | Male | |||
Tissue | lung | |||
Disease name | carcinoma | |||
Classification | cancer | |||
Recombinant | recombinant | |||
Exogene | Capsid protein of human adenovirus | Lifespan | infinite | |
Morphology | epithelial-like | |||
Cellosaurus(Expasy) | CVCL_E066 | |||
Medium | Medium List | Culture type | Adherent cells | |
Culture medium | DMEM (low glucose) + 10mM HEPES + 10% FBS + 0.1mM NEAA | |||
Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
Temperature | 37 ℃ | |||
CO2 concentration | 5 % | |||
Freeze medium | Medium + 10% DMSO | |||
Freezing method | Slow freezing | |||
Mycoplasma | (-) | |||
Isozyme | LD, NP | |||
STR(human) | OK | |||
Images | ||||
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Reference information | Reference | 2 | ||
User's Publication | 1 | |||