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Cell No. : Cell Name
RCB2352 : 633  update : 2023/10/16
CommentHuman lung cancer cell line expressing capsid protein of human adenovirus.
Comment from the depositor
Terms and conditions1) The BIOLOGICAL RESOURCE is provided as a service to research cpmmunity.2) A permission of the approver (Dr.Glen Nemerow & Scripps Research Institute) is required.
Remarks
approver's address
×
English
Address
10550 North Torrey Pines Road Immunology Building R214 Imm19 La Jolla, California 92037
Dr.Nemerow, Glen
Fax. +1-858-784-9910
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Yokoyama, K. & Nakamura, Y.
Originator Nemerow, Glen
Year of deposit 2006
Original cell A549
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue lung
Disease name carcinoma
Classification cancer
Recombinant recombinant
Exogene Capsid protein of human adenovirus
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_E066
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10mM HEPES + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
5182  Von Seggern DJ1, Huang S, Fleck SK, Stevenson SC, Nemerow GR.  Adenovirus vector pseudotyping in fiber-expressing cell lines: improved transduction of Epstein-Barr virus-transformed B cells.  J Virol  2000  74(1):354-62  PubMed ID: 10590124   DOI: 10.1128/jvi.74.1.354-362.2000
5183  Von Seggern DJ1, Kehler J, Endo RI, Nemerow GR.  Complementation of a fibre mutant adenovirus by packaging cell lines stably expressing the adenovirus type 5 fibre protein.  J Gen Virol  1998  79 ( Pt 6):1461-8  PubMed ID: 9634089   DOI: 10.1099/0022-1317-79-6-1461

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x


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