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Cell No. : Cell Name
RCB2350 : StromaNKtert  update : 2023/10/16
CommentHuman cell line derived from bone marrow stromal cells. Useful to propagate hematopoietic stem cells.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Blood 2003;101:532-540) .
approver's address
Contact Us
RIKEN, BioResource Research Center
Cell Engineering Division
Dr.HAMADA Hirofumi (Cell Bank)
Fax. +81-29-836-9130
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Hamada, Hirofumi
Originator Hamada, Hirofumi
Year of deposit 2006
Animal _human < Mammals
Genus Homo
Species sapiens
Tissue bone marrow
Classification transformed
Recombinant recombinant
Exogene hTERT, MFG-tsT-IRES-neo
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4667
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 12.5% FBS + 12.5% HS (GIBCO) + 1μM Hydrocortisone + 0.1mM 2-Mercaptoethanol
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 27

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15660  Kawano Y, Kobune M, Yamaguchi M, Nakamura K, Ito Y, Sasaki K, Takahashi S, Nakamura T, Chiba H, Sato T, Matsunaga T, Azuma H, Ikebuchi K, Ikeda H, Kato J, Niitsu Y, Hamada H.  Ex vivo expansion of human umbilical cord hematopoietic progenitor cells using a coculture system with human telomerase catalytic subunit (hTERT)-transfected human stromal cells  Blood  2003  101(2):532-40  PubMed ID: 12393449   DOI: 10.1182/blood-2002-04-1268

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User's Publication
19669  Parvin S, Aryal A, Yin S, Fell GG, Davids MS, Wu CJ, Letai A.  Targeting conditioned media dependencies and FLT-3 in chronic lymphocytic leukemia  Blood Adv  2023  7(19):5877-5889  PubMed ID: 37428863   DOI: 10.1182/bloodadvances.2022008207
17718  Muhowski EM, Ravikrishnan J, Gordon B, Yu L, Misra S, Walker B, Eathiraj S, Sampath D, Rogers KA, Byrd JC, Woyach JA.  Preclinical evaluation of combination nemtabrutinib and venetoclax in chronic lymphocytic leukemia  J Hematol Oncol  2022  15(1):166  PubMed ID: 36380319   DOI: 10.1186/s13045-022-01386-1
13511  Herbaux C, Kornauth C, Poulain S, Chong SJF, Collins MC, Valentin R, Hackett L, Tournilhac O, Lemonnier F, Dupuis J, Daniel A, Tomowiak C, Laribi K, Renaud L, Roos-Weil D, Rossi C, Van Den Neste EW, Leyronnas C, Merabet F, Malfuson JV, Tiab M, Ysebaert L, Ng SY, Morschhauser F, Staber PB, Davids MS.  BH3 profiling identifies ruxolitinib as a promising partner for venetoclax to treat T-cell prolymphocytic leukemia  Blood  2021  blood.2020007303  PubMed ID: 33598678   DOI: 10.1182/blood.2020007303
15651  Chen R, Tsai J, Thompson PA, Chen Y, Xiong P, Liu C, Burrows F, Sivina M, Burger JA, Keating MJ, Wierda WG, Plunkett W.  The multi-kinase inhibitor TG02 induces apoptosis and blocks B-cell receptor signaling in chronic lymphocytic leukemia through dual mechanisms of action  Blood Cancer J  2021  11(3):57  PubMed ID: 33714981   DOI: 10.1038/s41408-021-00436-0
15656  Herbst SA, Stolarczyk M, Becirovic T, Czernilofsky F, Liu Y, Kolb C, Knoll M, Herling M, Müller-Tidow C, Dietrich S.  Phagocytosis by stroma confounds coculture studies  iScience  2021  24(9):10306  PubMed ID: 34585113   DOI: 10.1016/j.isci.2021.103062
13375  Chong SJF, Iskandar K, Lai JXH, Qu J, Raman D, Valentin R, Herbaux C, Collins M, Low ICC, Loh T, Davids M, Pervaiz S.  Serine-70 phosphorylated Bcl-2 prevents oxidative stress-induced DNA damage by modulating the mitochondrial redox metabolism.  Nucleic Acids Res  2020    PubMed ID: 33245769   DOI: 10.1093/nar/gkaa1110
14794  Yi X, Sarkar A, Kismali G, Aslan B, Ayres M, Iles LR, Keating MJ, Wierda WG, Long JP, Bertilaccio MTS, Gandhi V.  AMG-176, an Mcl-1 Antagonist, Shows Preclinical Efficacy in Chronic Lymphocytic Leukemia  Clin Cancer Res  2020  26(14):3856-3867  PubMed ID: 31937611   DOI: 10.1158/1078-0432.CCR-19-1397
14299  Recasens-Zorzo C, Cardesa-Salzmann T, Petazzi P, Ros-Blanco L, Esteve-Arenys A, Clot G, Guerrero-Hernández M, Rodríguez V, Soldini D, Valera A, Moros A, Climent F, González-Barca E, Mercadal S, Arenillas L, Calvo X, Mate JL, Gutiérrez-García G, Casanova I, Mangues R, Sanjuan-Pla A, Bueno C, Menéndez P, Martínez A, Colomer D, Tejedor RE, Teixidó J, Campo E, López-Guillermo A, Borrell JI, Colomo L, Pérez-Galán P, Roué G.  Pharmacological modulation of CXCR4 cooperates with BET bromodomain inhibition in diffuse large B-cell lymphoma  Haematologica  2019  104(4):778-788  PubMed ID: 29954928   DOI: 10.3324/haematol.2017.180505
4352  Ding L, Zhang W, Yang L, Pelicano H, Zhou K, Yin R, Huang R, Zeng J.  Targeting the autophagy in bone marrow stromal cells overcomes resistance to vorinostat in chronic lymphocytic leukemia.  Onco Targets Ther  2018  11:5151-5170  PubMed ID: 30210236   DOI: 10.2147/OTT.S170392
14918  Esteve-Arenys A, Valero JG, Chamorro-Jorganes A, Gonzalez D, Rodriguez V, Dlouhy I, Salaverria I, Campo E, Colomer D, Martinez A, Rymkiewicz G, Pérez-Galán P, Lopez-Guillermo A, Roué G.  The BET bromodomain inhibitor CPI203 overcomes resistance to ABT-199 (venetoclax) by downregulation of BFL-1/A1 in in vitro and in vivo models of MYC+/BCL2+ double hit lymphoma  Oncogene  2018  37(14):1830-1844  PubMed ID: 29353886   DOI: 10.1038/s41388-017-0111-1
9948  Barile E, Marconi GD, De SK, Baggio C, Gambini L, Salem AF, Kashyap MK, Castro JE, Kipps TJ, Pellecchia M.  hBfl-1/hNOXA Interaction Studies Provide New Insights on the Role of Bfl-1 in Cancer Cell Resistance and for the Design of Novel Anticancer Agents.  ACS Chem. Biol.  2017  12:444-455  PubMed ID: 28026162   DOI: 10.1021/acschembio.6b00962
4552  Deng J, Isik E, Fernandes SM, Brown JR, Letai A, Davids MS.  Bruton's tyrosine kinase inhibition increases BCL-2 dependence and enhances sensitivity to venetoclax in chronic lymphocytic leukemia.  Leukemia  2017  31(10):2075-2084  PubMed ID: 28111464   DOI: 10.1038/leu.2017.32
15654  Kashyap MK, Amaya-Chanaga CI, Kumar D, Simmons B, Huser N, Gu Y, Hallin M, Lindquist K, Yafawi R, Choi MY, Amine AA, Rassenti LZ, Zhang C, Liu SH, Smeal T, Fantin VR, Kipps TJ, Pernasetti F, Castro JE.  Targeting the CXCR4 pathway using a novel anti-CXCR4 IgG1 antibody (PF-06747143) in chronic lymphocytic leukemia  J Hematol Oncol  2017  10(1):112  PubMed ID: 28526063   DOI: 10.1186/s13045-017-0435-x
13477  Balakrishnan K, Fu M, Onida F, Wierda WG, Keating MJ, Gandhi V.  Reactivation of Smac-mediated apoptosis in chronic lymphocytic leukemia cells: mechanistic studies of Smac mimetic  Oncotarget  2016  7(26):39458-39472  PubMed ID: 27223062   DOI: 10.18632/oncotarget.8462
15659  Chen JY, Lai YS, Tsai HJ, Kuo CC, Yen BL, Yeh SP, Sun HS, Hung WC.  The oncometabolite R-2-hydroxyglutarate activates NF-κB-dependent tumor-promoting stromal niche for acute myeloid leukemia cells  Sci Rep  2016  6:32428  PubMed ID: 27577048   DOI: 10.1038/srep32428
14285  Patel V, Balakrishnan K, Keating MJ, Wierda WG, Gandhi V.  Expression of executioner procaspases and their activation by a procaspase-activating compound in chronic lymphocytic leukemia cells  Blood  2015  125(7):1126-36  PubMed ID: 25538042   DOI: 10.1182/blood-2014-01-546796
14390  Zaman S, Shentu S, Yang J, He J, Orlowski RZ, Stellrecht CM, Gandhi V.  Targeting the pro-survival protein MET with tivantinib (ARQ 197) inhibits growth of multiple myeloma cells  Neoplasia  2015  17(3):289-300  PubMed ID: 25810013   DOI: 10.1016/j.neo.2015.01.006
15001  Balakrishnan K, Peluso M, Fu M, Rosin NY, Burger JA, Wierda WG, Keating MJ, Faia K, O'Brien S, Kutok JL, Gandhi V.  The phosphoinositide-3-kinase (PI3K)-delta and gamma inhibitor, IPI-145 (Duvelisib), overcomes signals from the PI3K/AKT/S6 pathway and promotes apoptosis in CLL  Leukemia  2015  29(9):1811-22  PubMed ID: 25917267   DOI: 10.1038/leu.2015.105
14394  Balakrishnan K, Burger JA, Fu M, Doifode T, Wierda WG, Gandhi V.  Regulation of Mcl-1 expression in context to bone marrow stromal microenvironment in chronic lymphocytic leukemia  Neoplasia  2014  16(12):1036-46  PubMed ID: 25499217   DOI: 10.1016/j.neo.2014.10.002
14395  El-Mabhouh AA, Ayres ML, Shpall EJ, Baladandayuthapani V, Keating MJ, Wierda WG, Gandhi V.  Evaluation of bendamustine in combination with fludarabine in primary chronic lymphocytic leukemia cells  Blood  2014  123(24):3780-9  PubMed ID: 24747434   DOI: 10.1182/blood-2013-12-541433
15655  Patel V, Chen LS, Wierda WG, Balakrishnan K, Gandhi V.  Impact of bone marrow stromal cells on Bcl-2 family members in chronic lymphocytic leukemia  Leuk Lymphoma  2014  55(4):899-910  PubMed ID: 23837491   DOI: 10.3109/10428194.2013.819573
15661  Fiorcari S, Brown WS, McIntyre BW, Estrov Z, Maffei R, O'Brien S, Sivina M, Hoellenriegel J, Wierda WG, Keating MJ, Ding W, Kay NE, Lannutti BJ, Marasca R, Burger JA.  The PI3-kinase delta inhibitor idelalisib (GS-1101) targets integrin-mediated adhesion of chronic lymphocytic leukemia (CLL) cell to endothelial and marrow stromal cells  PLoS One  2013  8(12):e83830  PubMed ID: 24376763   DOI: 10.1371/journal.pone.0083830
15649  Sivina M, Hartmann E, Vasyutina E, Boucas JM, Breuer A, Keating MJ, Wierda WG, Rosenwald A, Herling M, Burger JA.  Stromal cells modulate TCL1 expression, interacting AP-1 components and TCL1-targeting micro-RNAs in chronic lymphocytic leukemia  Leukemia  2012  26(8):1812-20  PubMed ID: 22460735   DOI: 10.1038/leu.2012.63
15650  Davids MS, Deng J, Wiestner A, Lannutti BJ, Wang L, Wu CJ, Wilson WH, Brown JR, Letai A.  Decreased mitochondrial apoptotic priming underlies stroma-mediated treatment resistance in chronic lymphocytic leukemia  Blood  2012  120(17):3501-9  PubMed ID: 22955911   DOI: 10.1182/blood-2012-02-414060
17207  Nakamori Y, Liu B, Ohishi K, Suzuki K, Ino K, Matsumoto T, Masuya M, Nishikawa H, Shiku H, Hamada H, Katayama N.  Human bone marrow stromal cells simultaneously support B and T/NK lineage development from human haematopoietic progenitors: a principal role for flt3 ligand in lymphopoiesis  Br J Haematol  2012  157(6):674-86  PubMed ID: 22463758   DOI: 10.1111/j.1365-2141.2012.09109.x
15652  Chen R, Guo L, Chen Y, Jiang Y, Wierda WG, Plunkett W.  Homoharringtonine reduced Mcl-1 expression and induced apoptosis in chronic lymphocytic leukemia  Blood  2011  117(1):156-64  PubMed ID: 20971952   DOI: 10.1182/blood-2010-01-262808
15653  Manshouri T, Estrov Z, Quintás-Cardama A, Burger J, Zhang Y, Livun A, Knez L, Harris D, Creighton CJ, Kantarjian HM, Verstovsek S.  Bone marrow stroma-secreted cytokines protect JAK2(V617F)-mutated cells from the effects of a JAK2 inhibitor  Cancer Res  2011  71(11):3831-40  PubMed ID: 21512135   DOI: 10.1158/0008-5472.CAN-10-4002

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