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Cell No. : Cell Name
RCB2347 : NMS-2  update : 2023/10/16
CommentHuman cell line derived from malignant peripheral nerve sheath tumor.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Niigata University

1-757, Asahimachi-dori, Niigata-Shi, Niigata-Ken, 951-8510 Japan
Dr.KAWASHIMA Hiroyuki
Fax. +81-25-227-0782
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kawashima, Hiroyuki
Originator Imaizumi, Satoshi
Year of deposit 2006
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 30 years
Tissue right thigh
Disease name malignant peripheral nerve sheath tumour
Metastatic ability Yes
Metastatic tissue peritoneum
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4662
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1 x 10 6 cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 3


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Reference
891  Hatano H, Tokunaga K, Ogose A, Imaizumi S, Hayami T, Yamagiwa H, Hotta T, Endo N, Takahashi HE, Naito M.  Origin of histiocyte-like cells and multinucleated giant cells in malignant fibrous histiocytoma: neoplastic or reactive?  Pathol Int  1999  49(1):14-22  PubMed ID: 10227720   DOI: 10.1046/j.1440-1827.1999.00819.x
890  Imaizumi S, Motoyama T, Ogose A, Hotta T, Takahashi HE.  Characterization and chemosensitivity of two human malignant peripheral nerve sheath tumour cell lines derived from a patient with neurofibromatosis type 1  Virchows Arch  1988  433(5):435-41  PubMed ID: 9849858   DOI: 10.1007/s004280050271

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User's Publication
17971  Magallón-Lorenz M, Terribas E, Ortega-Bertran S, Creus-Bachiller E, Fernández M, Requena G, Rosas I, Mazuelas H, Uriarte-Arrazola I, Negro A, Lausová T, Castellanos E, Blanco I, DeVries G, Kawashima H, Legius E, Brems H, Mautner V, Kluwe L, Ratner N, Wallace M, Fernández-Rodriguez J, Lázaro C, Fletcher JA, Reuss D, Carrió M, Gel B, Serra E.  Deep genomic analysis of malignant peripheral nerve sheath tumor cell lines challenges current malignant peripheral nerve sheath tumor diagnosis  iScience  2023  26(2):106096  PubMed ID: 36818284   DOI: 10.1016/j.isci.2023.106096
10608  Uotani K, Fujiwara T, Yoshida A, Iwata S, Morita T, Kiyono M, Yokoo S, Kunisada T, Takeda K, Hasei J, Numoto K, Nezu Y, Yonemoto T, Ishii T, Kawai A, Ochiya T, Ozaki T.  Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma.  Sci Rep  2017  7:14634  PubMed ID: 29116117   DOI: 10.1038/s41598-017-12660-5
17973  Itani S, Kunisada T, Morimoto Y, Yoshida A, Sasaki T, Ito S, Ouchida M, Sugihara S, Shimizu K, Ozaki T.  MicroRNA-21 correlates with tumorigenesis in malignant peripheral nerve sheath tumor (MPNST) via programmed cell death protein 4 (PDCD4)  J Cancer Res Clin Oncol  2012  138(9):1501-9  PubMed ID: 22526161   DOI: 10.1007/s00432-012-1223-1


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