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Cell No. : Cell Name
RCB2347 : NMS-2  update : 2018/07/06
CommentHuman cell line derived from malignant peripheral nerve sheath tumor.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Niigata University

1-757, Asahimachi-dori, Niigata-Shi, Niigata-Ken, 951-8510 Japan
Dr.Kawashima, Hiroyuki
Fax. +81-25-227-0782
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Kawashima, Hiroyuki
Originator Imaizumi, Satoshi
Year of deposit 2006
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 30 years
Tissue right thigh
Case history malignant peripheral nerve sheath tumour
Metastastatic ability Yes
Metastatsd tissue peritoneum
Classification cancer
Year of origin 1994
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 1 x 10^(6) cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 1

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5179  Imaizumi S, Motoyama T, Ogose A, Hotta T, Takahashi HE.  Characterization and chemosensitivity of two human malignant peripheral nerve sheath tumour cell lines derived from a patient with neurofibromatosis type 1.  Virchows Arch  1998  433(5):435-41  PubMed ID: 9849858

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User's Publication
10608  Uotani K, Fujiwara T, Yoshida A, Iwata S, Morita T, Kiyono M, Yokoo S, Kunisada T, Takeda K, Hasei J, Numoto K, Nezu Y, Yonemoto T, Ishii T, Kawai A, Ochiya T, Ozaki T.  Circulating MicroRNA-92b-3p as a Novel Biomarker for Monitoring of Synovial Sarcoma.  Sci Rep  2017  7:14634  PubMed ID: 29116117

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