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Cell No. : Cell Name
RCB2346 : NMFH-1  update : 2021/05/20
CommentHuman cell line derived from myxofibrosarcoma.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
approver's address
Niigata University

1-757, Asahimachi-dori, Niigata-Shi, Niigata-Ken, 951-8510 Japan
Fax. +81-25-227-0782
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kawashima, Hiroyuki
Originator Kawashima, Hiroyuki
Year of deposit 2006
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 89 years
Tissue left knee
Disease name myxofibrosarcoma
Classification cancer
Year of origin 1998
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4661
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 1 x 10^(6) cells/100 mm dish
Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
deposit info
lot info
Reference information Reference 1
User's Publication 3

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5178  Kawashima H, Ogose A, Gu W, Nishio J, Kudo N, Kondo N, Hotta T, Umezu H, Tohyama T, Nishijima H, Iwasaki H, Endo N.  Establishment and characterization of a novel myxofibrosarcoma cell line.  Cancer Genet Cytogenet  2005  161(1):28-35  PubMed ID: 16080955   DOI: 10.1016/j.cancergencyto.2005.02.003

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User's Publication
20416  Hu C, Yang L, Wang Y, Zhou S, Luo J, Gu Y.  Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop.  J Ginseng Res  2021  45(6):734-743  PubMed ID: 34764728   DOI: 10.1016/j.jgr.2021.05.004
12076  Kawarazaki A, Horinaka M, Yasuda S, Kawashima H, Numajiri T, Sakai T.  The HDAC inhibitor OBP-801 suppresses the growth of myxofibrosarcoma cells.  J BUON  2020    PubMed ID: 32277670  
14821  Shiraishi D, Fujiwara Y, Horlad H, Saito Y, Iriki T, Tsuboki J, Cheng P, Nakagata N, Mizuta H, Bekki H, Nakashima Y, Oda Y, Takeya M, Komohara Y.  CD163 Is Required for Protumoral Activation of Macrophages in Human and Murine Sarcoma  Cancer Res  2018  78(12):3255-3266  PubMed ID: 29610117   DOI: 10.1158/0008-5472.CAN-17-2011

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