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Cell No. : Cell Name
RCB2345 : EEB  update : 2024/01/17
CommentHuman cell line derived from erythroblastic leukemia.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref.(Leuk Res 2006;30:829-39) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Muroi, Kazuo
Originator Muroi, Kazuo
Year of deposit 2006
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 49 years
Tissue peripheral blood
Disease name early erythroblastic leukemia
Classification cancer
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_8685
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium StemSpan SFEM + 15% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 2


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Reference
5177  Kawano-Yamamoto C, Muroi K, Nagatsuka Y, Higuchi M, Kikuchi S, Nagai T, Hakomori SI, Ozawa K.  Establishment and characterization of a new erythroblastic leukemia cell line, EEB: phosphatidylglucoside-mediated erythroid differentiation and apoptosis.  Leuk Res  2006  30(7):829-39  PubMed ID: 16332389   DOI: 10.1016/j.leukres.2005.10.021

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User's Publication
19600  Adell MAY, Klockner TC, Höfler R, Wallner L, Schmid J, Markovic A, Martyniak A, Campbell CS.  Adaptation to spindle assembly checkpoint inhibition through the selection of specific aneuploidies  Genes Dev  2023  37(5-6):171-190  PubMed ID: 36859339   DOI: 10.1101/gad.350182.122
11768  Cazzola A, Schlegel C, Jansen I, Bochtler T, Jauch A, Krämer A.  TP53 deficiency permits chromosome abnormalities and karyotype heterogeneity in acute myeloid leukemia.  Leukemia  2019    PubMed ID: 31444400   DOI: 10.1038/s41375-019-0550-5



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