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Cell No. : Cell Name
RCB2344 : NCR-G4  update : 2022/01/26
CommentHuman cell line derived from embryonal carcinoma of testis.
Comment from the depositor
Terms and conditionsn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Acta Histchem. Cytochem.1992;25:562-576) .
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Basic information Depositor Hata, Jun-ichi
Originator Hata, Jun-ichi
Year of deposit 2006
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue testis
Disease name embryonal carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3062
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Medium Medium List
Culture type Adherent cells
Medium and additives G-031101 (with serum)
Antibiotics within G-031101
Passage method pipetting
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1 (or Medium + 10% DMSO)
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
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Reference information Reference 1
User's Publication 0

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948  J Hata, H Fujita, E Ikeda, Y Matsubayashi, Y Kokai, J Fujimoto  Differentiation of human germ cell tumor cells  Hum Cell  1989  2(4):382-7  PubMed ID: 2562098

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