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Cell No. : Cell Name
RCB2343 : NCR-G3  update : 2022/01/28
CommentHuman cell line derived from embryonal carcinoma of testis.Application consideration
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Acta Histchem. Cytochem.1992;25:562-576) .
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hata, Jun-ichi
Originator Hata, Jun-ichi
Year of deposit 2006
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue testis
Disease name embryonal carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3061
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives G-031101 (with serum)
Antibiotics within G-031101
Passage method pipetting
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : 3 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1 (or Medium + 10% DMSO)
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
deposit info
lot info
Reference information Reference 4
User's Publication 0


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Reference
8027  Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.  Lectin microarray analysis of pluripotent and multipotent stem cells.  Genes Cells  2011  16:1-11  PubMed ID: 21155951
4439  Ban Sato, Yohko U Katagiri, Kenji Miyado, Hidenori Akutsu, Yoshitaka Miyagawa, Yasuomi Horiuchi, Hideki Nakajima, Hajime Okita, Akihiro Umezawa, Jun-Ichi Hata, Junichiro Fujimoto, Kiyotaka Toshimori, Nobutaka Kiyokawa  Preferential localization of SSEA-4 in interfaces between blastomeres of mouse preimplantaion embryos  Biochem Biophys Res Commun  2007  364(4):838-43  PubMed ID: 18273440
4403  T Nakano, A Umezawa, H Abe, N Suzuki, T Yamada, S Nozawa, J Hata  A monoclonal antibody that specifically reacts with human embryonal carcinomas, spermatogonia and oocytes is able to induce human EC cell death  Differentiation  1995  58(3):233-40  PubMed ID: 7713330
948  J Hata, H Fujita, E Ikeda, Y Matsubayashi, Y Kokai, J Fujimoto  Differentiation of human germ cell tumor cells  Hum Cell  1989  2(4):382-7  PubMed ID: 2562098

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