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Cell No. : Cell Name
RCB2339 : XR-V15B  update : 2022/08/18
CommentA subline of the V79 cell line. X-ray-sensitive mutant. XRCC5(ku80) gene deficient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Zdzienicka, Malgorzata Z.
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3271
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 8
User's Publication 0


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Reference
5176  Li PC, Lam E, Roos WP, Zdzienicka MZ, Kaina B, Efferth T.  Artesunate derived from traditional Chinese medicine induces DNA damage and repair.  Cancer Res  2008  68(11):4347-51  PubMed ID: 18519695   DOI: 10.1158/0008-5472.CAN-07-2970
5175  Haveman J, Castro Kreder N, Rodermond HM, van Bree C, Franken NA, Stalpers LJ, Zdzienicka MZ, Peters GJ.  Cellular response of X-ray sensitive hamster mutant cell lines to gemcitabine, cisplatin and 5-fluorouracil.  Oncol Rep  2004  12(1):187-92  PubMed ID: 15201982   DOI: 10.3892/or.12.1.187
5170  Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G.  Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants.  Mol Cell Biol  1996  16(4):1519-26  PubMed ID: 8657125   DOI: 10.1128/mcb.16.4.1519
5174  Helbig R, Zdzienicka MZ, Speit G.  The effect of defective DNA double-strand break repair on mutations and chromosome aberrations in the Chinese hamster cell mutant XR-V15B.  Radiat Res  1995  143(2):151-7  PubMed ID: 7631007  
5173  Hafezparast M, Kaur GP, Zdzienicka M, Athwal RS, Lehmann AR, Jeggo PA.  Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36.  Somat Cell Mol Genet  1993  19(5):413-21  PubMed ID: 8291019  
5166  Zdzienicka MZ, van Wessel N, van der Schans GP.  A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.  Radiat Res  1992  131(3):309-14  PubMed ID: 1438689  
5172  Jeggo PA, Hafezparast M, Thompson AF, Broughton BC, Kaur GP, Zdzienicka MZ, Athwal RS.  Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.  Proc Natl Acad Sci U S A  1992  89(14):6423-7  PubMed ID: 1631138   DOI: 10.1073/pnas.89.14.6423
5171  Zdzienicka MZ, Tran Q, van der Schans GP, Simons JW.  Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells.  Mutat Res  1988  194(3):239-49  PubMed ID: 2460764   DOI: 10.1016/0167-8817(88)90025-9

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