Cell No. : Cell Name
RCB2339 : XR-V15B
update : 2023/10/16
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| Comment | A subline of the V79 cell line. X-ray-sensitive mutant. XRCC5(ku80) gene deficient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Zdzienicka, Malgorzata Z.
|
| Year of deposit |
2006
|
| Animal |
_hamster
< Mammals
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| Strain name |
Chinese
|
| Gender |
Male
|
| Tissue |
lung
|
| Classification |
mutant
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|
Lifespan |
infinite
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| Morphology |
fibroblast-like
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| Cellosaurus(Expasy) |
CVCL_3271
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
8
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| User's Publication |
0
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| Reference |
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5176
Li PC, Lam E, Roos WP, Zdzienicka MZ, Kaina B, Efferth T.
Artesunate derived from traditional Chinese medicine induces DNA damage and repair.
Cancer Res
2008
68(11):4347-51
PubMed ID: 18519695
DOI: 10.1158/0008-5472.CAN-07-2970
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5175
Haveman J, Castro Kreder N, Rodermond HM, van Bree C, Franken NA, Stalpers LJ, Zdzienicka MZ, Peters GJ.
Cellular response of X-ray sensitive hamster mutant cell lines to gemcitabine, cisplatin and 5-fluorouracil.
Oncol Rep
2004
12(1):187-92
PubMed ID: 15201982
DOI: 10.3892/or.12.1.187
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5170
Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G.
Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants.
Mol Cell Biol
1996
16(4):1519-26
PubMed ID: 8657125
DOI: 10.1128/mcb.16.4.1519
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5174
Helbig R, Zdzienicka MZ, Speit G.
The effect of defective DNA double-strand break repair on mutations and chromosome aberrations in the Chinese hamster cell mutant XR-V15B.
Radiat Res
1995
143(2):151-7
PubMed ID: 7631007
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5173
Hafezparast M, Kaur GP, Zdzienicka M, Athwal RS, Lehmann AR, Jeggo PA.
Subchromosomal localization of a gene (XRCC5) involved in double strand break repair to the region 2q34-36.
Somat Cell Mol Genet
1993
19(5):413-21
PubMed ID: 8291019
DOI: 10.1007/BF01233246
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5166
Zdzienicka MZ, van Wessel N, van der Schans GP.
A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.
Radiat Res
1992
131(3):309-14
PubMed ID: 1438689
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5172
Jeggo PA, Hafezparast M, Thompson AF, Broughton BC, Kaur GP, Zdzienicka MZ, Athwal RS.
Localization of a DNA repair gene (XRCC5) involved in double-strand-break rejoining to human chromosome 2.
Proc Natl Acad Sci U S A
1992
89(14):6423-7
PubMed ID: 1631138
DOI: 10.1073/pnas.89.14.6423
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5171
Zdzienicka MZ, Tran Q, van der Schans GP, Simons JW.
Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells.
Mutat Res
1988
194(3):239-49
PubMed ID: 2460764
DOI: 10.1016/0167-8817(88)90025-9
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