Cell No. : Cell Name
RCB2338 : XR-V9B-4
update : 2024/01/24
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| Comment | A subline of the V79B cell line. X-ray-sensitive mutant. XRCC5(ku80) gene deficient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Zdzienicka, Malgorzata Z.
|
| Year of deposit |
2006
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Gender |
Male
|
| Tissue |
lung
|
| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_8651
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
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Free
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|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
|
5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
|
OK
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| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
4
|
| User's Publication |
0
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| Reference |
|
5188
van Duijn-Goedhart A, Zdzienicka MZ, Sankaranarayanan K, van Buul PP.
Differential responses of Chinese hamster mutagen sensitive cell lines to low and high concentrations of calicheamicin and neocarzinostatin.
Mutat Res
2000
471(1-2):95-105
PubMed ID: 11080665
DOI: 10.1016/s1383-5718(00)00122-4
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5170
Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G.
Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants.
Mol Cell Biol
1996
16(4):1519-26
PubMed ID: 8657125
DOI: 10.1128/mcb.16.4.1519
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5160
Pergola F, Zdzienicka MZ, Lieber MR.
V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.
Mol Cell Biol
1993
13(6):3464-71
PubMed ID: 8497262
DOI: 10.1128/mcb.13.6.3464
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5166
Zdzienicka MZ, van Wessel N, van der Schans GP.
A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.
Radiat Res
1992
131(3):309-14
PubMed ID: 1438689
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