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Cell No. : Cell Name
RCB2338 : XR-V9B-4  update : 2022/06/29
CommentA subline of the V79B cell line. X-ray-sensitive mutant. XRCC5(ku80) gene deficient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Zdzienicka, Malgorzata Z.
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8651
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 4
User's Publication 0


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Reference
5188  van Duijn-Goedhart A, Zdzienicka MZ, Sankaranarayanan K, van Buul PP.  Differential responses of Chinese hamster mutagen sensitive cell lines to low and high concentrations of calicheamicin and neocarzinostatin.  Mutat Res  2000  471(1-2):95-105  PubMed ID: 11080665   DOI: 10.1016/s1383-5718(00)00122-4
5170  Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G.  Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants.  Mol Cell Biol  1996  16(4):1519-26  PubMed ID: 8657125   DOI: 10.1128/mcb.16.4.1519
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262   DOI: 10.1128/mcb.13.6.3464
5166  Zdzienicka MZ, van Wessel N, van der Schans GP.  A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.  Radiat Res  1992  131(3):309-14  PubMed ID: 1438689  

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