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Cell No. : Cell Name
RCB2337 : V79B  update : 2021/05/20
CommentA subline of the V79 cell line. Widely used for radiation biology, mutagenesis and mammalian cell genetics.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Zdzienicka, Malgorzata Z.
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6296
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 6
User's Publication 1


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Reference
5169  Drexler GA, Rogge S, Beisker W, Eckardt-Schupp F, Zdzienicka MZ, Fritz E.  Spontaneous homologous recombination is decreased in Rad51C-deficient hamster cells.  DNA Repair (Amst)  2004  3(10):1335-43  PubMed ID: 15336628  
5168  Becker R, Ritter A, Eichhorn U, Lips J, Bertram B, Wiessler M, Zdzienicka MZ, Kaina B.  Induction of DNA breaks and apoptosis in crosslink-hypersensitive V79 cells by the cytostatic drug beta-D-glucosyl-ifosfamide mustard.  Br J Cancer  2002  86(1):130-5  PubMed ID: 11857024   DOI: 10.1038/sj.bjc.6600027
5188  van Duijn-Goedhart A, Zdzienicka MZ, Sankaranarayanan K, van Buul PP.  Differential responses of Chinese hamster mutagen sensitive cell lines to low and high concentrations of calicheamicin and neocarzinostatin.  Mutat Res  2000  471(1-2):95-105  PubMed ID: 11080665   DOI: 10.1016/s1383-5718(00)00122-4
5167  Telleman P, Overkamp WJ, van Wessel N, Studzian K, Wetselaar L, Natarajan AT, Zdzienicka MZ.  A new complementation group of mitomycin C-hypersensitive Chinese hamster cell mutants that closely resembles the phenotype of fanconi anemia cells.  Cancer Res  1995  55(15):3412-6  PubMed ID: 7614481  
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262   DOI: 10.1128/mcb.13.6.3464
5166  Zdzienicka MZ, van Wessel N, van der Schans GP.  A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.  Radiat Res  1992  131(3):309-14  PubMed ID: 1438689  

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User's Publication
20077  Muhammad H, Omar MH, Rasid ENI, Suhaimi SN, Mohkiar FH, Siu LM, Awang N.  Phytochemical and In Vitro Genotoxicity Studies of Standardized Ficus deltoidea var. kunstleri Aqueous Extract.  Plants (Basel)  2021  10(2)  PubMed ID: 33670296   DOI: 10.3390/plants10020343



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