Cell No. : Cell Name
RCB2336 : V-G8
update : 2023/10/16
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| Comment | A subline of the V79 cell line. X-ray-sensitive mutant. XRCC8 gene deficient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Zdzienicka, Malgorzata Z.
|
| Year of deposit |
2006
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Gender |
Male
|
| Tissue |
lung
|
| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_T046
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
8
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| User's Publication |
0
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| Reference |
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5164
Jongmans W, Verhaegh GW, Jaspers NG, Demant P, Natarajan AT, Shiloh Y, Oshimura M, Stanbridge EJ, Athwal RS, Cuthbert AP, Newbold RF, Lohman PH, Zdzienicka MZ.
The defect in the AT-like hamster cell mutants is complemented by mouse chromosome 9 but not by any of the human chromosomes.
Mutat Res
1996
364(2):91-102
PubMed ID: 8879275
DOI: 10.1016/0921-8777(96)00027-4
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5159
Verhaegh GW, Jaspers NG, Lohman PH, Zdzienicka MZ.
Co-dominance of radioresistant DNA synthesis in a group of AT-like Chinese hamster cell mutants.
Cytogenet Cell Genet
1993
63(3):176-80
PubMed ID: 8485992
DOI: 10.1159/000133528
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5160
Pergola F, Zdzienicka MZ, Lieber MR.
V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.
Mol Cell Biol
1993
13(6):3464-71
PubMed ID: 8497262
DOI: 10.1128/mcb.13.6.3464
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5161
Jongmans W, Verhaegh GW, Sankaranarayanan K, Lohman PH, Zdzienicka MZ.
Cellular characteristics of Chinese hamster cell mutants resembling ataxia telangiectasia cells.
Mutat Res
1993
294(3):207-14
PubMed ID: 7692260
DOI: 10.1016/0921-8777(93)90003-y
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5162
Jongmans W, Wiegant J, Oshimura M, James MR, Lohman PH, Zdzienicka MZ.
Human chromosome 11 complements ataxia-telangiectasia cells but does not complement the defect in AT-like Chinese hamster cell mutants.
Hum Genet
1993
92(3):259-64
PubMed ID: 8406433
DOI: 10.1007/bf00244469
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5158
Zdzienicka MZ, Jaspers NG, van der Schans GP, Natarajan AT, Simons JW.
Ataxia-telangiectasia-like Chinese hamster V79 cell mutants with radioresistant DNA synthesis, chromosomal instability, and normal DNA strand break repair.
Cancer Res
1989
49(6):1481-5
PubMed ID: 2466555
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5171
Zdzienicka MZ, Tran Q, van der Schans GP, Simons JW.
Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells.
Mutat Res
1988
194(3):239-49
PubMed ID: 2460764
DOI: 10.1016/0167-8817(88)90025-9
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5157
Zdzienicka MZ, Simons JW.
Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese hamster cells.
Mutat Res
1987
178(2):235-44
PubMed ID: 3587254
DOI: 10.1016/0027-5107(87)90274-0
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