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Cell No. : Cell Name
RCB2335 : V-E5  update : 2022/08/18
CommentX-ray-sensitive mutant derived from the V79 cell line. XRCC8 gene deficient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Zdzienicka, Malgorzata Z.
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification mutant
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T045
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 10
User's Publication 0


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Reference
5165  Sultana R, McNeill DR, Abbotts R, Mohammed MZ, Zdzienicka MZ, Qutob H, Seedhouse C, Laughton CA, Fischer PM, Patel PM, Wilson DM 3rd, Madhusudan S.  Synthetic lethal targeting of DNA double-strand break repair deficient cells by human apurinic/apyrimidinic endonuclease inhibitors.  Int J Cancer  2012  131(10):2433-44  PubMed ID: 22377908   DOI: 10.1002/ijc.27512
5188  van Duijn-Goedhart A, Zdzienicka MZ, Sankaranarayanan K, van Buul PP.  Differential responses of Chinese hamster mutagen sensitive cell lines to low and high concentrations of calicheamicin and neocarzinostatin.  Mutat Res  2000  471(1-2):95-105  PubMed ID: 11080665   DOI: 10.1016/s1383-5718(00)00122-4
5164  Jongmans W, Verhaegh GW, Jaspers NG, Demant P, Natarajan AT, Shiloh Y, Oshimura M, Stanbridge EJ, Athwal RS, Cuthbert AP, Newbold RF, Lohman PH, Zdzienicka MZ.  The defect in the AT-like hamster cell mutants is complemented by mouse chromosome 9 but not by any of the human chromosomes.  Mutat Res  1996  364(2):91-102  PubMed ID: 8879275   DOI: 10.1016/0921-8777(96)00027-4
5163  Helbig R, Gerland E, Zdzienicka MZ, Speit G.  The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.  Mutat Res  1995  336(3):307-16  PubMed ID: 7739617   DOI: 10.1016/0921-8777(95)00005-5
5159  Verhaegh GW, Jaspers NG, Lohman PH, Zdzienicka MZ.  Co-dominance of radioresistant DNA synthesis in a group of AT-like Chinese hamster cell mutants.  Cytogenet Cell Genet  1993  63(3):176-80  PubMed ID: 8485992   DOI: 10.1159/000133528
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262   DOI: 10.1128/mcb.13.6.3464
5161  Jongmans W, Verhaegh GW, Sankaranarayanan K, Lohman PH, Zdzienicka MZ.  Cellular characteristics of Chinese hamster cell mutants resembling ataxia telangiectasia cells.  Mutat Res  1993  294(3):207-14  PubMed ID: 7692260   DOI: 10.1016/0921-8777(93)90003-y
5162  Jongmans W, Wiegant J, Oshimura M, James MR, Lohman PH, Zdzienicka MZ.  Human chromosome 11 complements ataxia-telangiectasia cells but does not complement the defect in AT-like Chinese hamster cell mutants.  Hum Genet  1993  92(3):259-64  PubMed ID: 8406433   DOI: 10.1007/bf00244469
5158  Zdzienicka MZ, Jaspers NG, van der Schans GP, Natarajan AT, Simons JW.  Ataxia-telangiectasia-like Chinese hamster V79 cell mutants with radioresistant DNA synthesis, chromosomal instability, and normal DNA strand break repair.  Cancer Res  1989  49(6):1481-5  PubMed ID: 2466555  
5157  Zdzienicka MZ, Simons JW.  Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese hamster cells.  Mutat Res  1987  178(2):235-44  PubMed ID: 3587254   DOI: 10.1016/0027-5107(87)90274-0

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