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Cell No. : Cell Name
RCB2332 : V79-4  update : 2021/05/20
CommentA subline of the V79 cell line following cloning. Widely used for radiation biology, mutagenesis and mammalian cell genetics.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Ernest H. Y. Chu
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Gender Male
Tissue lung
Classification other
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2796
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 6
User's Publication 0


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Reference
5156  Popescu NC, Amsbaugh SC, DiPaolo JA.  Human and rodent transformed cells are more sensitive to in vitro induction of SCE by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) than normal cells.  Hum Genet  1983  63(1):53-7  PubMed ID: 6299927  
5155  Popescu NC, Amsbaugh SC, Larramendy ML, DiPaolo JA.  12-O-tetradecanoyl-phorbol-13-acetate and its relationship to SCE induction in Syrian and Chinese hamster cells.  Environ Mutagen  1982  4(1):73-81  PubMed ID: 7067669  
5153  Myhr BC, Dipaolo JA.  Mutagenesis by N-acetoxy-2-acetyl aminofluorene of chinese hamster V79 cells is unaffected by caffeine.  Chem Biol Interact  1978  21(1):1-18  PubMed ID: 566636  
5154  Popescu NC, Turnbull D, DiPaolo JA.  Sister chromatid exchange and chromosome aberration analysis with the use of several carcinogens and noncarcinogens.  J Natl Cancer Inst  1977  59(1):289-93  PubMed ID: 406414   DOI: 10.1093/jnci/59.1.289
5152  Myhr BC, Dipalo JA.  Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells.  Genetics  1975  80(1):157-69  PubMed ID: 1093934  
5151  Chu EH, Malling HV.  Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.  Proc Natl Acad Sci U S A  1968  61(4):1306-12  PubMed ID: 5249812  

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