Cell No. : Cell Name
RCB2332 : V79-4
update : 2023/10/16
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| Comment | A subline of the V79 cell line following cloning. Widely used for radiation biology, mutagenesis and mammalian cell genetics. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Ernest H. Y. Chu
|
| Year of deposit |
2006
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Gender |
Male
|
| Tissue |
lung
|
| Classification |
other
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2796
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
|
| User's Publication |
0
|
| Reference |
|
5156
Popescu NC, Amsbaugh SC, DiPaolo JA.
Human and rodent transformed cells are more sensitive to in vitro induction of SCE by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) than normal cells.
Hum Genet
1983
63(1):53-7
PubMed ID: 6299927
DOI: 10.1007/BF00285398
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5155
Popescu NC, Amsbaugh SC, Larramendy ML, DiPaolo JA.
12-O-tetradecanoyl-phorbol-13-acetate and its relationship to SCE induction in Syrian and Chinese hamster cells.
Environ Mutagen
1982
4(1):73-81
PubMed ID: 7067669
DOI: 10.1002/em.2860040110
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5153
Myhr BC, Dipaolo JA.
Mutagenesis by N-acetoxy-2-acetyl aminofluorene of chinese hamster V79 cells is unaffected by caffeine.
Chem Biol Interact
1978
21(1):1-18
PubMed ID: 566636
DOI: 10.1016/0009-2797(78)90064-9
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5154
Popescu NC, Turnbull D, DiPaolo JA.
Sister chromatid exchange and chromosome aberration analysis with the use of several carcinogens and noncarcinogens.
J Natl Cancer Inst
1977
59(1):289-93
PubMed ID: 406414
DOI: 10.1093/jnci/59.1.289
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5152
Myhr BC, Dipalo JA.
Requirement for cell dispersion prior to selection of induced azaguanine-resistant colonies of Chinese hamster cells.
Genetics
1975
80(1):157-69
PubMed ID: 1093934
DOI: 10.1093/genetics/80.1.157
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5151
Chu EH, Malling HV.
Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.
Proc Natl Acad Sci U S A
1968
61(4):1306-12
PubMed ID: 5249812
DOI: 10.1073/pnas.61.4.1306
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