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Cell No. : Cell Name
RCB2331 : XR-1  update : 2021/05/20
CommentA subline of the CHO-K1 cell line. X-ray-sensitive mutant. XRCC4 gene deficient.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Room 227, 100 East Lancaster Avenue, Wynnewood PA 19096
Dr.Stamato, Thomas D.
Fax. +81-+1-610-645-2205
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Stamato, Thomas D.
Year of deposit 2006
Original cell CHO-K1
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_K253
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 11
User's Publication 0


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Reference
5150  Morrish TA, Garcia-Perez JL, Stamato TD, Taccioli GE, Sekiguchi J, Moran JV.  Endonuclease-independent LINE-1 retrotransposition at mammalian telomeres.  Nature  2007  446(7132):208-12  PubMed ID: 17344853
5149  Delacôte F, Han M, Stamato TD, Jasin M, Lopez BS.  An xrcc4 defect or Wortmannin stimulates homologous recombination specifically induced by double-strand breaks in mammalian cells.  Nucleic Acids Res  2002  30(15):3454-63  PubMed ID: 12140331
5148  Bryans M, Valenzano MC, Stamato TD.  Absence of DNA ligase IV protein in XR-1 cells: evidence for stabilization by XRCC4.  Mutat Res  1999  433(1):53-8  PubMed ID: 10047779
5147  Li Z, Otevrel T, Gao Y, Cheng HL, Seed B, Stamato TD, Taccioli GE, Alt FW.  The XRCC4 gene encodes a novel protein involved in DNA double-strand break repair and V(D)J recombination.  Cell  1995  83(7):1079-89  PubMed ID: 8548796
5163  Helbig R, Gerland E, Zdzienicka MZ, Speit G.  The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.  Mutat Res  1995  336(3):307-16  PubMed ID: 7739617
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262
5166  Zdzienicka MZ, van Wessel N, van der Schans GP.  A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.  Radiat Res  1992  131(3):309-14  PubMed ID: 1438689
5145  Giaccia AJ1, MacLaren RA, Denko N, Nicolaou D, Stamato TD.  Increased sensitivity to killing by restriction enzymes in the XR-1 DNA double-strand break repair-deficient mutant.  Mutat Res  1990  236(1):67-76  PubMed ID: 2164147
5146  Giaccia AJ, Denko N, MacLaren R, Mirman D, Waldren C, Hart I, Stamato TD.  Human chromosome 5 complements the DNA double-strand break-repair deficiency and gamma-ray sensitivity of the XR-1 hamster variant.  Am J Hum Genet  1990  47(3):459-69  PubMed ID: 1697445
5144  Giaccia AJ, Richardson E, Denko N, Stamato TD.  Genetic analysis of XR-1 mutation in hamster and human hybrids.  Somat Cell Mol Genet  1989  15(1):71-7  PubMed ID: 2916163
5143  Stamato TD, Weinstein R, Giaccia A, Mackenzie L.  Isolation of cell cycle-dependent gamma ray-sensitive Chinese hamster ovary cell.  Somatic Cell Genet  1983  9(2):165-73  PubMed ID: 6836453

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