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Cell No. : Cell Name
RCB2330 : CHO-K1  update : 2020/09/24
CommentChinese hamster ovary cell line. Widely used for the isolation of mutants by mutagenesis.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Miyoshi, Hiroyuki
Originator Theodore T. Puck
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification other
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
4176  Mushirobira Y, Nishimiya O, Nagata J, Todo T, Hara A, Reading BJ, Hiramatsu N.  Molecular cloning of vitellogenin gene promoters and in vitro and in vivo transcription profiles following estradiol-17β administration in the cutthroat trout.  Gen. Comp. Endocrinol.  2018    PubMed ID: 29966659



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