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Cell No. : Cell Name
RCB2329 : V-3  update : 2022/08/18
CommentA subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC7(DNA-PK) gene deficient.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the following literature (Int J Radiat Biol 1989;56: 657-665) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator G. F. Whitmore
Year of deposit 2006
Original cell CHO-AA8
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_K039
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 5
User's Publication 0


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Reference
5163  Helbig R, Gerland E, Zdzienicka MZ, Speit G.  The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.  Mutat Res  1995  336(3):307-16  PubMed ID: 7739617   DOI: 10.1016/0921-8777(95)00005-5
5142  Taccioli GE, Cheng HL, Varghese AJ, Whitmore G, Alt FW.  A DNA repair defect in Chinese hamster ovary cells affects V(D)J recombination similarly to the murine scid mutation.  J Biol Chem  1994  269(10):7439-42  PubMed ID: 8125964  
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262   DOI: 10.1128/mcb.13.6.3464
5166  Zdzienicka MZ, van Wessel N, van der Schans GP.  A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.  Radiat Res  1992  131(3):309-14  PubMed ID: 1438689  
5141  Whitmore GF, Varghese AJ, Gulyas S.  Cell cycle responses of two X-ray sensitive mutants defective in DNA repair.  Int J Radiat Biol  1989  56(5):657-65  PubMed ID: 2573661   DOI: 10.1080/09553008914551881

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