Cell No. : Cell Name
RCB2329 : V-3
update : 2025/06/26
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| Comment | A subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC7(DNA-PK) gene deficient. |
| Comment from the depositor | |
| Terms and conditions | In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the following literature （Int J Radiat Biol 1989;56: 657-665) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyoshi, Hiroyuki
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| Originator |
G. F. Whitmore
|
| Year of deposit |
2006
|
| Original cell |
CHO-AA8
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
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| Genus |
Cricetulus
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| Species |
griseus
|
| Gender |
Female
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| Age at sampling |
adult
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| Tissue |
ovary
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| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_K039
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM ALPHA + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Animal PCR |
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OK
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| DNA barcoding |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
6
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| User's Publication |
0
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| Reference |
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5163
Helbig R, Gerland E, Zdzienicka MZ, Speit G.
The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.
Mutat Res
1995
336(3):307-16
PubMed ID: 7739617
DOI: 10.1016/0921-8777(95)00005-5
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5142
Taccioli GE, Cheng HL, Varghese AJ, Whitmore G, Alt FW.
A DNA repair defect in Chinese hamster ovary cells affects V(D)J recombination similarly to the murine scid mutation.
J Biol Chem
1994
269(10):7439-42
PubMed ID: 8125964
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5160
Pergola F, Zdzienicka MZ, Lieber MR.
V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.
Mol Cell Biol
1993
13(6):3464-71
PubMed ID: 8497262
DOI: 10.1128/mcb.13.6.3464
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5166
Zdzienicka MZ, van Wessel N, van der Schans GP.
A fourth complementation group among ionizing radiation-sensitive Chinese hamster cell mutants defective in DNA double-strand break repair.
Radiat Res
1992
131(3):309-14
PubMed ID: 1438689
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5141
Whitmore GF, Varghese AJ, Gulyas S.
Cell cycle responses of two X-ray sensitive mutants defective in DNA repair.
Int J Radiat Biol
1989
56(5):657-65
PubMed ID: 2573661
DOI: 10.1080/09553008914551881
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2586
Puck, T. T. et al
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.
J Exp Med
1958
108:945
PubMed ID: 13598821
DOI: 10.1084/jem.108.6.945
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