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Cell No. : Cell Name
RCB2328 : irs1SF  update : 2021/05/20
CommentA subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC3 gene deficient.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of thefollowing literature (Mutat Res 1988;193:109-121) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Robert B. Painter
Year of deposit 2006
Original cell CHO-AA8
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_K038
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 8
User's Publication 0


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Reference
5175  Haveman J, Castro Kreder N, Rodermond HM, van Bree C, Franken NA, Stalpers LJ, Zdzienicka MZ, Peters GJ.  Cellular response of X-ray sensitive hamster mutant cell lines to gemcitabine, cisplatin and 5-fluorouracil.  Oncol Rep  2004  12(1):187-92  PubMed ID: 15201982   DOI: 10.3892/or.12.1.187
5163  Helbig R, Gerland E, Zdzienicka MZ, Speit G.  The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.  Mutat Res  1995  336(3):307-16  PubMed ID: 7739617   DOI: 10.1016/0921-8777(95)00005-5
5139  Tucker JD1, Jones NJ, Allen NA, Minkler JL, Thompson LH, Carrano AV.  Cytogenetic characterization of the ionizing radiation-sensitive Chinese hamster mutant irs1.  Mutat Res  1991  254(2):143-52  PubMed ID: 1900570   DOI: 10.1016/0921-8777(91)90005-a
5134  Jones NJ, Stewart SA, Thompson LH.  Biochemical and genetic analysis of the Chinese hamster mutants irs1 and irs2 and their comparison to cultured ataxia telangiectasia cells.  Mutagenesis  1990  5(1):15-23  PubMed ID: 2158612   DOI: 10.1093/mutage/5.1.15
5137  Jones NJ1, Cox R, Thacker J.  Six complementation groups for ionising-radiation sensitivity in Chinese hamster cells.  Mutat Res  1988  193(2):139-44  PubMed ID: 3347206   DOI: 10.1016/0167-8817(88)90044-2
5138  Debenham PG1, Jones NJ, Webb MB.  Vector-mediated DNA double-strand break repair analysis in normal, and radiation-sensitive, Chinese hamster V79 cells.  Mutat Res  1988  199(1):1-9  PubMed ID: 2834642   DOI: 10.1016/0027-5107(88)90224-2
5140  Fuller LF, Painter RB.  A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication.  Mutat Res  1988  193(2):109-21  PubMed ID: 3347204   DOI: 10.1016/0167-8817(88)90041-7
2204  Jones, N J, Cox R, Thacker J  Isolation and cross-sensitivity of X-ray-sensitive mutants of V79-4 hamster cells.  Mutat Res  1987  183:279-86  PubMed ID: 3106801   DOI: 10.1016/0167-8817(87)90011-3

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