Cell No. : Cell Name
RCB2327 : EM-9
update : 2025/06/25
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| Comment | A subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC1 gene deficient. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Larry H. Thompson
|
| Year of deposit |
2006
|
| Original cell |
CHO-AA8
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Genus |
Cricetulus
|
| Species |
griseus
|
| Gender |
Female
|
| Age at sampling |
adult
|
| Tissue |
ovary
|
| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_3683
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM ALPHA + 10% FBS
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| Antibiotics |
|
Free
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Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
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| Animal PCR |
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OK
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| DNA barcoding |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
9
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| User's Publication |
0
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| Reference |
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5163
Helbig R, Gerland E, Zdzienicka MZ, Speit G.
The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.
Mutat Res
1995
336(3):307-16
PubMed ID: 7739617
DOI: 10.1016/0921-8777(95)00005-5
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2124
Thompson LH, Brookman KW, Jones NJ, Allen SA, Carrano AV.
Molecular cloning of the human XRCC1 gene, which corrects defective DNA strand break repair and sister chromatid exchange
Mol Cell Biol
1990
10(12):6160-71
PubMed ID: 2247054
DOI: 10.1128/mcb.10.12.6160-6171.1990
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5133
Thompson LH, Brookman KW, Minkler JL, Fuscoe JC, Henning KA, Carrano AV.
DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange.
Mol Cell Biol
1985
5(4):881-4
PubMed ID: 3990694
DOI: 10.1128/mcb.5.4.881
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5136
Chan JY, Thompson LH, Becker FF.
DNA-ligase activities appear normal in the CHO mutant EM9.
Mutat Res
1984
131(5-6):209-14.
PubMed ID: 6738565
DOI: 10.1016/0167-8817(84)90027-0
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5132
La Belle M, Linn S, Thompson LH.
Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9.
Mutat Res
1984
141(1):41-4.
PubMed ID: 6482894
DOI: 10.1016/0165-7992(84)90035-6
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5128
Dillehay LE, Thompson LH, Minkler JL, Carrano AV.
The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.
Mutat Res
1983
109(2):283-96
PubMed ID: 6843572
DOI: 10.1016/0027-5107(83)90053-2
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5126
Thompson LH, Brookman KW, Dillehay LE, Carrano AV, Mazrimas JA, Mooney CL, Minkler JL.
A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
Mutat Res
1982
95(2-3):427-40
PubMed ID: 6889677
DOI: 10.1016/0027-5107(82)90276-7
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5124
Thompson LH, Rubin JS, Cleaver JE, Whitmore GF, Brookman K.
A screening method for isolating DNA repair-deficient mutants of CHO cells.
Somatic Cell Genet
1980
6(3):391-405.
PubMed ID: 7404270
DOI: 10.1007/BF01542791
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2586
Puck, T. T. et al
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.
J Exp Med
1958
108:945
PubMed ID: 13598821
DOI: 10.1084/jem.108.6.945
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