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Cell No. : Cell Name
RCB2327 : EM-9 update : 2024/02/01 |
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| Comment | A subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC1 gene deficient. | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Miyoshi, Hiroyuki | ||
| Originator | Larry H. Thompson | |||
| Year of deposit | 2006 | |||
| Original cell | CHO-AA8 | |||
| Animal | _hamster < Mammals | |||
| Strain name | Chinese | |||
| Genus | Cricetulus | |||
| Species | griseus | |||
| Gender | Female | |||
| Age at sampling | adult | |||
| Tissue | ovary | |||
| Classification | mutant | |||
| Lifespan | infinite | |||
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_3683 | |||
| Medium | Medium List | |||
| Culture type | Adherent cells | |||
| Culture medium | MEM ALPHA + 10% FBS | |||
| Antibiotics | Free | |||
| Passage method | 0.25% Trypsin | |||
| Culture information | Passage ratio | 1 : 8 split | ||
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 5 | ||
| User's Publication | 0 | |||
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| Reference |
| 5163 Helbig R, Gerland E, Zdzienicka MZ, Speit G. The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5. Mutat Res 1995 336(3):307-16 PubMed ID: 7739617 DOI: 10.1016/0921-8777(95)00005-5 |
| 5133 Thompson LH, Brookman KW, Minkler JL, Fuscoe JC, Henning KA, Carrano AV. DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange. Mol Cell Biol 1985 5(4):881-4 PubMed ID: 3990694 DOI: 10.1128/mcb.5.4.881 |
| 5136 Chan JY, Thompson LH, Becker FF. DNA-ligase activities appear normal in the CHO mutant EM9. Mutat Res 1984 131(5-6):209-14. PubMed ID: 6738565 DOI: 10.1016/0167-8817(84)90027-0 |
| 5132 La Belle M, Linn S, Thompson LH. Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9. Mutat Res 1984 141(1):41-4. PubMed ID: 6482894 DOI: 10.1016/0165-7992(84)90035-6 |
| 5128 Dillehay LE, Thompson LH, Minkler JL, Carrano AV. The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells. Mutat Res 1983 109(2):283-96 PubMed ID: 6843572 DOI: 10.1016/0027-5107(83)90053-2 |
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