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Cell No. : Cell Name
RCB2327 : EM-9  update : 2022/08/18
CommentA subline of the CHO-K1 cell line. X-ray-sensitive mutant derived from CHO-AA8 cell line. XRCC1 gene deficient.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Larry H. Thompson
Year of deposit 2006
Original cell CHO-AA8
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_3683
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 5
User's Publication 0


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Reference
5163  Helbig R, Gerland E, Zdzienicka MZ, Speit G.  The pattern of mutations induced by neocarzinostatin and methyl methanesulfonate in the ataxia telangiectasia-like Chinese hamster cell line V-E5.  Mutat Res  1995  336(3):307-16  PubMed ID: 7739617   DOI: 10.1016/0921-8777(95)00005-5
5133  Thompson LH, Brookman KW, Minkler JL, Fuscoe JC, Henning KA, Carrano AV.  DNA-mediated transfer of a human DNA repair gene that controls sister chromatid exchange.  Mol Cell Biol  1985  5(4):881-4  PubMed ID: 3990694   DOI: 10.1128/mcb.5.4.881
5136  Chan JY, Thompson LH, Becker FF.  DNA-ligase activities appear normal in the CHO mutant EM9.  Mutat Res  1984  131(5-6):209-14.  PubMed ID: 6738565   DOI: 10.1016/0167-8817(84)90027-0
5132  La Belle M, Linn S, Thompson LH.  Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9.  Mutat Res  1984  141(1):41-4.  PubMed ID: 6482894   DOI: 10.1016/0165-7992(84)90035-6
5128  Dillehay LE, Thompson LH, Minkler JL, Carrano AV.  The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.  Mutat Res  1983  109(2):283-96  PubMed ID: 6843572   DOI: 10.1016/0027-5107(83)90053-2

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