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Cell No. : Cell Name
RCB2326 : CHO-AA8  update : 2021/05/20
CommentA subline of the CHO-K1 cell line. Widely used for the isolation of repair-deficient mutants. Useful for gene mutation assays at the APRT and HPRT loci.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyoshi, Hiroyuki
Originator Larry H. Thompson
Year of deposit 2006
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification mutant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4386
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
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lot info
Reference information Reference 12
User's Publication 3


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Reference
5160  Pergola F, Zdzienicka MZ, Lieber MR.  V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.  Mol Cell Biol  1993  13(6):3464-71  PubMed ID: 8497262   DOI: 10.1128/mcb.13.6.3464
5140  Fuller LF, Painter RB.  A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication.  Mutat Res  1988  193(2):109-21  PubMed ID: 3347204   DOI: 10.1016/0167-8817(88)90041-7
5129  Thompson LH, Brookman KW, Mooney CL.  Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene.  Somat Cell Mol Genet  1984  10(2):183-94  PubMed ID: 6584989  
5130  Dillehay LE, Thompson LH, Carrano AV.  DNA-strand breaks associated with halogenated pyrimidine incorporation.  Mutat Res  1984  131(3-4):129-36  PubMed ID: 6717468   DOI: 10.1016/0167-8817(84)90052-x
5136  Chan JY, Thompson LH, Becker FF.  DNA-ligase activities appear normal in the CHO mutant EM9.  Mutat Res  1984  131(5-6):209-14.  PubMed ID: 6738565   DOI: 10.1016/0167-8817(84)90027-0
5131  MacInnes MA, Bingham JM, Thompson LH, Strniste GF.  DNA-mediated cotransfer of excision repair capacity and drug resistance into chinese hamster ovary mutant cell line UV-135.  Mol Cell Biol  1984  4(6):1152-8  PubMed ID: 6738535   DOI: 10.1128/mcb.4.6.1152
5132  La Belle M, Linn S, Thompson LH.  Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9.  Mutat Res  1984  141(1):41-4.  PubMed ID: 6482894   DOI: 10.1016/0165-7992(84)90035-6
5128  Dillehay LE, Thompson LH, Minkler JL, Carrano AV.  The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.  Mutat Res  1983  109(2):283-96  PubMed ID: 6843572   DOI: 10.1016/0027-5107(83)90053-2
5126  Thompson LH, Brookman KW, Dillehay LE, Carrano AV, Mazrimas JA, Mooney CL, Minkler JL.  A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.  Mutat Res  1982  95(2-3):427-40  PubMed ID: 6889677   DOI: 10.1016/0027-5107(82)90276-7
5127  Thompson LH, Brookman KW, Dillehay LE, Mooney CL, Carrano AV.  Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions.  Somatic Cell Genet  1982  8(6):759-73  PubMed ID: 7163954  
5124  Thompson LH, Rubin JS, Cleaver JE, Whitmore GF, Brookman K.  A screening method for isolating DNA repair-deficient mutants of CHO cells.  Somatic Cell Genet  1980  6(3):391-405.  PubMed ID: 7404270  
5125  Thompson LH, Fong S, Brookman K.  Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells.  Mutat Res  1980  74(1):21-36  PubMed ID: 7360155  

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User's Publication
12984  Kawanishi M, Yoneda R, Totsuka Y, Yagi T.  Genotoxicity of micro- and nano-particles of kaolin in human primary dermal keratinocytes and fibroblasts.  Genes Environ  2020    PubMed ID: 32322315   DOI: 10.1186/s41021-020-00155-1
11932  Kawanishi M, Hisatomi Y, Oda Y, Shimohara C, Tsunematsu Y, Sato M, Hirayama Y, Miyoshi N, Iwashita Y, Yoshikawa Y, Sugimura H, Mutoh M, Ishikawa H, Wakabayashi K, Yagi T, Watanabe K.  In vitro genotoxicity analyses of colibactin-producing E. coli isolated from a Japanese colorectal cancer patient.  J Toxicol Sci  2019    PubMed ID: 31813906   DOI: 10.2131/jts.44.871
15932  Hirayama R, Ito A, Noguchi M, Matsumoto Y, Uzawa A, Kobashi G, Okayasu R, Furusawa Y.  OH radicals from the indirect actions of X-rays induce cell lethality and mediate the majority of the oxygen enhancement effect  Radiat Res  2013  180:514-23  PubMed ID: 24138483  



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