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Cell No. : Cell Name
RCB2324 : MIH5(RPMI)  update : 2022/10/19
CommentHybridoma producing a monoclonal antibody against mouse B7-H1. Possible to culture in RPMI1640 medium.
Comment from the depositor
Terms and conditions1) The RECIOIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent from the DEPOCITOR. 2) The RECIPIENT shall be a non-profit entity only. 3) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purposes only. 4) In publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR’s SCIENTIST is requested. Also the RECIPIENT agree to acknowledgment to the DEPOSITOR’s SCIENTIST as a coauthor in such publication that the BIOLOGICAL RESOURCE materially contributes to the publications. 5) if the RECIPIENT discovers creates or derives new material or invention though the use of the BIOLOGICAL RESOURCE, the ownership of any patent or other intellectual property right to the material or invention shall be negotiated between PROVIDER and RECIPIENT
approver's address
Graduate School, Tokyo Medical and Dental University

1-5-45, Yushima, Bunkyo-ku, Tokyo Japan
Dr.AZUMA Miyuki
Fax. +81-3-5803-5935
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Azuma, Miyuki
Originator Azuma, Miyuki
Year of deposit 2006
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C664
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain rat, SD
Fusion_cell_1 gender Female
Fusion_cell_2 name P3U1
Fusion_cell_2 strain mouse
Fusion method PEG, HAT selection
Antibody name MIH5
Subclass IgG2a
Immunogen mouse B7-H1 transfected L5178Y
Antigen_name B7-H1/PD-L1
Antigen_character mouse B7-H1
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
deposit info
lot info
Reference information Reference 0
User's Publication 1

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User's Publication
15451  Igarashi Y, Onoe T, Ohdan H.  The Role of Liver Sinusoidal Endothelial Cells in Induction of Carbohydrate Reactive B Cells Tolerance Through the Programmed Death 1/Programmed Death Ligand 1 Pathway  Transplantation  2015  99(11):2325-36  PubMed ID: 26247556   DOI: 10.1097/TP.0000000000000831

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