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Cell No. : Cell Name
RCB2323 : 9-15C  update : 2021/05/20
CommentMouse mesenchymal cell line derived from bone marrow. C3H/He
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2006
Animal mouse < Mammals
Strain name C3H/He
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_6532
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2-3 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 2


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Reference
8027  Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.  Lectin microarray analysis of pluripotent and multipotent stem cells.  Genes Cells  2011  16:1-11  PubMed ID: 21155951
6508  Yamada Y, Sakurada K, Takeda Y, Gojo S, Umezawa A.  Single-cell-derived mesenchymal stem cells overexpressing Csx/Nkx2.5 and GATA4 undergo the stochastic cardiomyogenic fate and behave like transient amplifying cells.  Exp Cell Res  2007  313(4):698-706  PubMed ID: 17208226

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User's Publication
15661  Fiorcari S, Brown WS, McIntyre BW, Estrov Z, Maffei R, O'Brien S, Sivina M, Hoellenriegel J, Wierda WG, Keating MJ, Ding W, Kay NE, Lannutti BJ, Marasca R, Burger JA.  The PI3-kinase delta inhibitor idelalisib (GS-1101) targets integrin-mediated adhesion of chronic lymphocytic leukemia (CLL) cell to endothelial and marrow stromal cells  PLoS One  2013  8(12):e83830  PubMed ID: 24376763
7732  Hoellenriegel J, Meadows SA, Sivina M, Wierda WG, Kantarjian H, Keating MJ, Giese N, O'Brien S, Yu A, Miller LL, Lannutti BJ, Burger JA.  The phosphoinositide 3'-kinase delta inhibitor, CAL-101, inhibits B-cell receptor signaling and chemokine networks in chronic lymphocytic leukemia.  Blood  2011  118:3603-12  PubMed ID: 21803855



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