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Cell No. : Cell Name
RCB2322 : KUM5 update : 2023/10/16 |
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| Comment | Mouse mesenchymal cell line derived from bone marrow. C3H/He | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Umezawa, Akihiro | ||
| Originator | Umezawa, Akihiro | |||
| Year of deposit | 2006 | |||
| Animal | _mouse < Mammals | |||
| Strain name | C3H/He | |||
| Tissue | bone marrow | |||
| Classification | normal | |||
| Lifespan | infinite | |||
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_5151 | |||
| Medium | Medium List | |||
| Culture type | Adherent cells | |||
| Culture medium | POWEREDBY10 | |||
| Antibiotics | Free | |||
| Passage method | 0.25% Trypsin | |||
| Culture information | Passage cell No | 2-3 x 10 5 cells/100 mm dish | ||
| SC frequency | Subculture : once/week, Medium Renewal : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| SSLP(mouse) | OK | |||
| Isozyme | LD, NP | |||
| Images | ||||
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| Reference information | Reference | 2 | ||
| User's Publication | 4 | |||
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| Reference |
| 8027 Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A. Lectin microarray analysis of pluripotent and multipotent stem cells. Genes Cells 2011 16:1-11 PubMed ID: 21155951 DOI: 10.1111/j.1365-2443.2010.01459.x |
| 2540 Toyoda M, Hamatani T, Okada H, Matsumoto K, Saito H, Umezawa A. Defining cell identity by comprehensive gene expression profiling Curr Med Chem 2010 17(28):3245-52 PubMed ID: 20666720 DOI: 10.2174/092986710792231932 |
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| User's Publication |
| 21493 Lp MRL, Agrawal DK. Biomechanical Forces in the Tissue Engineering and Regeneration of Shoulder, Hip, Knee, and Ankle Joints. J Biotechnol Biomed 2023 6(4):491-500 PubMed ID: 38037618 DOI: 10.26502/jbb.2642-91280111 |
| 14340 Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH. CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment Mol Cancer Ther 2021 PubMed ID: 34088832 DOI: 10.1158/1535-7163.MCT-20-0591 |
| 7285 Correia V, Panadero JA, Ribeiro C, Sencadas V, Rocha JG, Gomez Ribelles JL, Lanceros-Mendez S. Design and validation of a biomechanical bioreactor for cartilage tissue culture. Biomech Model Mechanobiol 2016 15:471-8 PubMed ID: 26153426 DOI: 10.1007/s10237-015-0698-5 |
| 7439 Panadero JA, Sencadas V, Silva SC, Ribeiro C, Correia V, Gama FM, Gomez Ribelles JL, Lanceros-Mendez S. Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus. J. Biomed. Mater. Res. Part B Appl. Biomater. 2016 104:330-8 PubMed ID: 25772257 DOI: 10.1002/jbm.b.33386 |
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