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Cell No. : Cell Name
RCB2322 : KUM5  update : 2021/05/20
CommentMouse mesenchymal cell line derived from bone marrow. C3H/He
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2006
Animal mouse < Mammals
Strain name C3H/He
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_5151
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 2-3 x 10^(5) cells/100 mm dish
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 3

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8027  Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.  Lectin microarray analysis of pluripotent and multipotent stem cells.  Genes Cells  2011  16:1-11  PubMed ID: 21155951   DOI: 10.1111/j.1365-2443.2010.01459.x
2540  Toyoda M, Hamatani T, Okada H, Matsumoto K, Saito H, Umezawa A.  Defining cell identity by comprehensive gene expression profiling  Curr Med Chem  2010  17(28):3245-52  PubMed ID: 20666720   DOI: 10.2174/092986710792231932

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User's Publication
14340  Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.  CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment  Mol Cancer Ther  2021    PubMed ID: 34088832   DOI: 10.1158/1535-7163.MCT-20-0591
7285  Correia V, Panadero JA, Ribeiro C, Sencadas V, Rocha JG, Gomez Ribelles JL, Lanceros-Mendez S.  Design and validation of a biomechanical bioreactor for cartilage tissue culture.  Biomech Model Mechanobiol  2016  15:471-8  PubMed ID: 26153426   DOI: 10.1007/s10237-015-0698-5
7439  Panadero JA, Sencadas V, Silva SC, Ribeiro C, Correia V, Gama FM, Gomez Ribelles JL, Lanceros-Mendez S.  Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus.  J. Biomed. Mater. Res. Part B Appl. Biomater.  2016  104:330-8  PubMed ID: 25772257   DOI: 10.1002/jbm.b.33386

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