Cell No. : Cell Name
RCB2322 : KUM5
update : 2023/10/16
|
| Comment | Mouse mesenchymal cell line derived from bone marrow. C3H/He |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
|
Basic information
|
Depositor |
Umezawa, Akihiro
|
| Originator |
Umezawa, Akihiro
|
| Year of deposit |
2006
|
| Animal |
_mouse
< Mammals
|
| Strain name |
C3H/He
|
| Tissue |
bone marrow
|
| Classification |
normal
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_5151
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
POWEREDBY10
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage cell No |
|
2-3 x 10 5 cells/100 mm dish
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| SSLP(mouse) |
|
OK
|
| Isozyme |
|
LD, NP
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
4
|
| Reference |
|
8027
Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.
Lectin microarray analysis of pluripotent and multipotent stem cells.
Genes Cells
2011
16:1-11
PubMed ID: 21155951
DOI: 10.1111/j.1365-2443.2010.01459.x
|
|
2540
Toyoda M, Hamatani T, Okada H, Matsumoto K, Saito H, Umezawa A.
Defining cell identity by comprehensive gene expression profiling
Curr Med Chem
2010
17(28):3245-52
PubMed ID: 20666720
DOI: 10.2174/092986710792231932
|
| User's Publication |
|
21493
Lp MRL, Agrawal DK.
Biomechanical Forces in the Tissue Engineering and Regeneration of Shoulder, Hip, Knee, and Ankle Joints.
J Biotechnol Biomed
2023
6(4):491-500
PubMed ID: 38037618
DOI: 10.26502/jbb.2642-91280111
|
|
14340
Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.
CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment
Mol Cancer Ther
2021
PubMed ID: 34088832
DOI: 10.1158/1535-7163.MCT-20-0591
|
|
7285
Correia V, Panadero JA, Ribeiro C, Sencadas V, Rocha JG, Gomez Ribelles JL, Lanceros-Mendez S.
Design and validation of a biomechanical bioreactor for cartilage tissue culture.
Biomech Model Mechanobiol
2016
15:471-8
PubMed ID: 26153426
DOI: 10.1007/s10237-015-0698-5
|
|
7439
Panadero JA, Sencadas V, Silva SC, Ribeiro C, Correia V, Gama FM, Gomez Ribelles JL, Lanceros-Mendez S.
Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus.
J. Biomed. Mater. Res. Part B Appl. Biomater.
2016
104:330-8
PubMed ID: 25772257
DOI: 10.1002/jbm.b.33386
|
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