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Cell No. : Cell Name
RCB2318 : P19.CL6  update : 2024/07/19
CommentMouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear efficiently.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Komuro, Issei
Originator Habara, Akemi
Year of deposit 2006
Another name CL6
Original cell P19
Animal _mouse < Mammals
Strain name C3H
Genus Mus
Species musculus
Gender Male
Age at sampling embryo
Tissue embryonal carcinoma
Classification cancer
History Originator -> Registor
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L981
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% Trypsin
Culture information Passage ratio 1 : 20 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 11

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3467  Peng, Chang-Fu, Wei, Yi, Levsky, Jeffrey M, McDonald, Thomas V, Childs, Geoffrey, Kitsis, Richard N  Microarray analysis of global changes in gene expression during cardiac myocyte differentiation.  Physiol Genomics  2002  9:145-55  PubMed ID: 12045295   DOI: 10.1152/physiolgenomics.00027.2002
2406  Habara-Ohkubo A.  Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells.  Cell Struct Funct  1996  21(2):101-10  PubMed ID: 8790939   DOI: 10.1247/csf.21.101
4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443   DOI: 10.1016/0012-1606(82)90338-4
4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336   DOI: 10.1038/299165a0

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User's Publication
20996  Morita T, Hayashi K.  Actin-related protein 5 suppresses the cooperative activation of cardiac gene transcription by myocardin and MEF2.  FEBS Open Bio  2023    PubMed ID: 36610028   DOI: 10.1002/2211-5463.13549
16365  Min KD, Asakura M, Shirai M, Yamazaki S, Ito S, Fu HY, Asanuma H, Asano Y, Minamino T, Takashima S, Kitakaze M.  ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis  Sci Rep  2021  11(1):23056  PubMed ID: 34845242   DOI: 10.1038/s41598-021-02390-0
6945  Uchida L, Tanaka T, Saito H, Sugahara M, Wakashima T, Fukui K, Nangaku M.  Effects of a prolyl hydroxylase inhibitor on kidney and cardiovascular complications in a rat model of chronic kidney disease.  Am J Physiol Renal Physiol  2020  318(2):F388-F401  PubMed ID: 31841388   DOI: 10.1152/ajprenal.00419.2019
7042  Kimura H, Eguchi S, Sasaki J, Kuba K, Nakanishi H, Takasuga S, Yamazaki M, Goto A, Watanabe H, Itoh H, Imai Y, Suzuki A, Mizushima N, Sasaki T.  Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy.  JCI Insight  2017  2:e89462  PubMed ID: 28097232   DOI: 10.1172/jci.insight.89462
14050  Yamaoka T, Hirata M, Dan T, Yamashita A, Otaka A, Nakaoki T, Miskon A, Kakinoki S, Mahara A.  Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates  J Biomed Mater Res A   2017  105(4):1166-1174  PubMed ID: 27977899   DOI: 10.1002/jbm.a.35977
14687  Micheletti R, Plaisance I, Abraham BJ, Sarre A, Ting CC, Alexanian M, Maric D, Maison D, Nemir M, Young RA, Schroen B, González A, Ounzain S, Pedrazzini T.  The long noncoding RNA Wisper controls cardiac fibrosis and remodeling  Sci Transl Med  2017  9(395):eaai9118  PubMed ID: 28637928   DOI: 10.1126/scitranslmed.aai9118
14758  Alexanian M, Maric D, Jenkinson SP, Mina M, Friedman CE, Ting CC, Micheletti R, Plaisance I, Nemir M, Maison D, Kernen J, Pezzuto I, Villeneuve D, Burdet F, Ibberson M, Leib SL, Palpant NJ, Hernandez N, Ounzain S, Pedrazzini T.  A transcribed enhancer dictates mesendoderm specification in pluripotency  Nat Commun  2017  8(1):1806.  PubMed ID: 29180618   DOI: 10.1038/s41467-017-01804-w
15158  Ounzain S, Micheletti R, Arnan C, Plaisance I, Cecchi D, Schroen B, Reverter F, Alexanian M, Gonzales C, Ng SY, Bussotti G, Pezzuto I, Notredame C, Heymans S, Guigó R, Johnson R, Pedrazzini T.  CARMEN, a human super enhancer-associated long noncoding RNA controlling cardiac specification, differentiation and homeostasis  J Mol Cell Cardiol  2015  89(Pt A):98-112  PubMed ID: 26423156   DOI: 10.1016/j.yjmcc.2015.09.016
15243  Kakinoki S, Seo JH, Inoue Y, Ishihara K, Yui N, Yamaoka T.  Mobility of the Arg-Gly-Asp ligand on the outermost surface of biomaterials suppresses integrin-mediated mechanotransduction and subsequent cell functions  Acta Biomater  2015  13:42-51  PubMed ID: 25463493   DOI: 10.1016/j.actbio.2014.11.020
15991  Dehne T, Adam X, Materne EM, Reimann MC, Krüger JP, Van Linthout S, Tschöpe C, Haag M, Sittinger M, Ringe J.  A P19 and P19CL6 cell-based complementary approach to determine paracrine effects in cardiac tissue engineering  Cells Tissues Organs  2014  199(1):24-36  PubMed ID: 25170977   DOI: 10.1159/000362540
16033  Yazaki Y, Oyane A, Araki H, Sogo Y, Ito A, Yamazaki A, Tsurushima H.  Fabrication of DNA-antibody-apatite composite layers for cell-targeted gene transfer  Sci Technol Adv Mater  2012  13(6):064204  PubMed ID: 27877531   DOI: 10.1088/1468-6996/13/6/064204

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