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Cell No. : Cell Name
RCB2318 : P19.CL6  update : 2021/12/21
CommentMouse cell line derived from embryonal carcinoma. C3H strain. Following differentiation, beating cardiomyocytes appear efficiently.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Komuro, Issei
Originator Habara, Akemi
Year of deposit 2006
Another name CL6
Original cell P19
Animal _mouse < Mammals
Strain name C3H
Genus Mus
Species musculus
Gender Male
Age at sampling embryo
Tissue embryonal carcinoma
Classification cancer
History Originator -> Registor
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_L981
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin
Culture information Passage ratio 1 : 20 split
SC frequency Subculture : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 4
User's Publication 7


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Reference
3467  Peng, Chang-Fu, Wei, Yi, Levsky, Jeffrey M, McDonald, Thomas V, Childs, Geoffrey, Kitsis, Richard N  Microarray analysis of global changes in gene expression during cardiac myocyte differentiation.  Physiol Genomics  2002  9:145-55  PubMed ID: 12045295
2406  Habara-Ohkubo A.  Differentiation of beating cardiac muscle cells from a derivative of P19 embryonal carcinoma cells.  Cell Struct Funct  1996  21(2):101-10  PubMed ID: 8790939
4962  McBurney MW, Rogers BJ.  Isolation of male embryonal carcinoma cells and their chromosome replication patterns.  Dev Biol  1982  89(2):503-8  PubMed ID: 7056443
4963  McBurney MW, Jones-Villeneuve EM, Edwards MK, Anderson PJ.  Control of muscle and neuronal differentiation in a cultured embryonal carcinoma cell line.  Nature  1982  299(5879):165-7  PubMed ID: 7110336

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User's Publication
6945  Uchida L, Tanaka T, Saito H, Sugahara M, Wakashima T, Fukui K, Nangaku M.  Effects of a prolyl hydroxylase inhibitor on kidney and cardiovascular complications in a rat model of chronic kidney disease.  Am J Physiol Renal Physiol  2020  318(2):F388-F401  PubMed ID: 31841388
7042  Kimura H, Eguchi S, Sasaki J, Kuba K, Nakanishi H, Takasuga S, Yamazaki M, Goto A, Watanabe H, Itoh H, Imai Y, Suzuki A, Mizushima N, Sasaki T.  Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy.  JCI Insight  2017  2:e89462  PubMed ID: 28097232
14050  Yamaoka T, Hirata M, Dan T, Yamashita A, Otaka A, Nakaoki T, Miskon A, Kakinoki S, Mahara A.  Individual evaluation of cardiac marker expression and self-beating during cardiac differentiation of P19CL6 cells on different culture substrates  J Biomed Mater Res A   2017  105(4):1166-1174  PubMed ID: 27977899
14687  Micheletti R, Plaisance I, Abraham BJ, Sarre A, Ting CC, Alexanian M, Maric D, Maison D, Nemir M, Young RA, Schroen B, González A, Ounzain S, Pedrazzini T.  The long noncoding RNA Wisper controls cardiac fibrosis and remodeling  Sci Transl Med  2017  9(395):eaai9118  PubMed ID: 28637928
14758  Alexanian M, Maric D, Jenkinson SP, Mina M, Friedman CE, Ting CC, Micheletti R, Plaisance I, Nemir M, Maison D, Kernen J, Pezzuto I, Villeneuve D, Burdet F, Ibberson M, Leib SL, Palpant NJ, Hernandez N, Ounzain S, Pedrazzini T.  A transcribed enhancer dictates mesendoderm specification in pluripotency  Nat Commun  2017  8(1):1806.  PubMed ID: 29180618
15158  Ounzain S, Micheletti R, Arnan C, Plaisance I, Cecchi D, Schroen B, Reverter F, Alexanian M, Gonzales C, Ng SY, Bussotti G, Pezzuto I, Notredame C, Heymans S, Guigó R, Johnson R, Pedrazzini T.  CARMEN, a human super enhancer-associated long noncoding RNA controlling cardiac specification, differentiation and homeostasis  J Mol Cell Cardiol  2015  89(Pt A):98-112  PubMed ID: 6423156
15243  Kakinoki S, Seo JH, Inoue Y, Ishihara K, Yui N, Yamaoka T.  Mobility of the Arg-Gly-Asp ligand on the outermost surface of biomaterials suppresses integrin-mediated mechanotransduction and subsequent cell functions  Acta Biomater  2015  13:42-51  PubMed ID: 25463493



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