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Cell No. : Cell Name
RCB2317 : AC1  update : 2022/01/21
CommentHybridoma producing monoclonal antibody against GD1c gangliosides.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochim Biophys Acta 1997;1345:207-214) designated by the DEPOSITOR is required.
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Basic information Depositor Nohara, Keiko
Originator Nohara, Keiko
Year of deposit 2006
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U859
hybridoma Fusion_cell_1 name PAI
Fusion_cell_1 strain mouse, BALB/c
Fusion_cell_2 name spleen
Fusion_cell_2 strain mouse, C3H/HeN
Fusion method PEG, HAT selection
Antibody name AC1
Subclass IgG3
Immunogen GD1c gangliosides (NeuGc, NeuGc)
Antigen_character GD1c gangliosides (NeuGc, NeuGc)
Antigen_positive mouse Tcell and rat Tcell
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
deposit info
lot info
Reference information Reference 1
User's Publication 0

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3616  Nohara K, Ozawa H, Tai T, Saji H, Fujimaki H.  Gangliosides involved in activation of rat T lineage cells.  Biochim Biophys Acta  1997  1345(2):207-14  PubMed ID: 9106500

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