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Cell No. : Cell Name
RCB2316 : AB1  update : 2022/01/21
CommentHybridoma producing monoclonal antibody against GD1b gangliosides.
Comment from the depositor
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochim Biophys Acta 1997;1345:207-214) designated by the DEPOSITOR is required.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Nohara, Keiko
Originator Nohara, Keiko
Year of deposit 2006
Animal mouse x mouse < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_U858
hybridoma Fusion_cell_1 name spleen
Fusion_cell_1 strain mouse, C3H/HeN
Fusion_cell_2 name PAI
Fusion_cell_2 strain mouse, BALB/c
Fusion method PEG, HAT selection
Antibody name AB1
Subclass IgG3
Immunogen GD1b gangliosides (NeuGc, NeuGc)
Antigen_character GD1b gangliosides (NeuGc, NeuGc)
Antigen_positive mouse Tcell and rat Tcell
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG3
Reference information Reference 1
User's Publication 0


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Reference
3616  Nohara K, Ozawa H, Tai T, Saji H, Fujimaki H.  Gangliosides involved in activation of rat T lineage cells.  Biochim Biophys Acta  1997  1345(2):207-14  PubMed ID: 9106500   DOI: 10.1016/s0005-2760(96)00181-6

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