Cell No. : Cell Name
RCB2316 : AB1
update : 2024/01/17
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| Comment | Hybridoma producing monoclonal antibody against GD1b gangliosides. |
| Comment from the depositor | |
| Terms and conditions | 1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochim Biophys Acta 1997;1345:207-214) designated by the DEPOSITOR is required. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Nohara, Keiko
|
| Originator |
Nohara, Keiko
|
| Year of deposit |
2006
|
| Animal |
_mouse x mouse
< Hybrid
|
| Tissue |
hybridoma
|
| Classification |
hybrid cells
|
|
Lifespan |
infinite
|
| Morphology |
lymphocyte-like
|
| Cellosaurus(Expasy) |
CVCL_U858
|
|
hybridoma
|
Fusion_cell_1 name |
spleen
|
| Fusion_cell_1 strain |
mouse, C3H/HeN
|
| Fusion_cell_2 name |
PAI
|
| Fusion_cell_2 strain |
mouse, BALB/c
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|
Fusion method |
PEG, HAT selection
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|
Antibody name |
AB1
|
| Subclass |
IgG3
|
| Immunogen |
GD1b gangliosides (NeuGc, NeuGc)
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|
Antigen_character |
GD1b gangliosides (NeuGc, NeuGc)
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|
Antigen_positive |
mouse Tcell and rat Tcell
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|
| |
deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Suspension cells
|
| Culture method |
|
浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
|
RPMI1640 + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
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Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
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Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
|
Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Antibody subclass |
|
IgG3
|
| Reference information |
Reference |
1
|
| User's Publication |
0
|
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