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Cell No. : Cell Name
RCB2314 : F9/LM10  update : 2023/10/16
CommentMouse cell line derived from embryonal carcinoma. Expressing laminin alpha-5 exogenously.
Comment from the depositor
Terms and conditions1) A permission of the approver is required. 2) The users should sign an MTA with Osaka University. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Matrix Biol 2006;25:85-88) designated by the DEPOSITOR is required.
approver's address
Institute for Protein Research, Osaka University
Laboratory of Extracellular Matrix Biochemistry
3-2 Yamadaoka, Suita, Osaka 565-0871 Japan
Dr.SEKIGUCHI Kiyotoshi
Fax. +81-6-6879-8619
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sekiguchi, Kiyotoshi
Originator Hayashi, Yoshitake (Nagoya Univ.)
Year of deposit 2006
Original cell F9
Animal _mouse < Mammals
Strain name 129
Gender Male
Age at sampling embryo
Tissue embryo
Disease name Embryonal carcinoma
Classification cancer
Recombinant recombinant
Exogene Laminin α5 cDNA, puromycine resistant
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_T373
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Coating dish 0.1% gelatin coated dish
Culture information Passage ratio 1 : 8 split
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 0

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3562  Hayashi Y, Weber CN, Emoto T, Fujiwara H, Sanzen N, Futaki S, Sekiguchi K.  A novel large-scale production system for modified basement membrane matrices using gene-swapped parietal endoderm cells.  Matrix Biol  2006  25(2):85-8  PubMed ID: 16314080   DOI: 10.1016/j.matbio.2005.10.004
2493  Strickland S, Mahdavi V.  The induction of differentiation in teratocarcinoma stem cells by retinoic acid.  Cell  1978  15(2):393-403  PubMed ID: 214238   DOI: 10.1016/0092-8674(78)90008-9

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