Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB2312 : 859H5  update : 2021/05/20
CommentHybridoma producing monoclonal antibody against bacterial unknown molecule. Rat IgG2a monoclonal antibody.
Comment from the depositor
Terms and conditions1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 1999;19:3918-3925) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takayama, Hajime
Originator Fujita, Shinobu
Year of deposit 2006
Animal mouse x rat < Hybrid
Tissue hybridoma
Classification hybrid cells
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_C477
hybridoma Fusion_cell_1 name P3-X63-Ag8.653
Fusion_cell_1 strain mouse
Fusion_cell_2 name spleen cell
Fusion_cell_2 strain rat, Wistar
Fusion method PEG
Antibody name 859H5
Subclass IgG2a
Immunogen bacterial unknown molecule
Antigen_name bacterial unknown molecule
Antigen_m.w. 70kDa
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Antibody subclass IgG2a
Reference information Reference 1
User's Publication 0


To topTop
Reference
3615      J Neurochem  1999  19:3918-392   

To topTop
User's Publication



Back Back Return Top Page